Rapp Mikaela, Drew David, Daley Daniel O, Nilsson Johan, Carvalho Tiago, Melén Karin, De Gier Jan-Willem, Von Heijne Gunnar
Department of Medical Biochemistry and Biophysics, Karolinska Institutet, SE-171 77 Stockholm, Sweden.
Protein Sci. 2004 Apr;13(4):937-45. doi: 10.1110/ps.03553804.
Membrane protein topology predictions can be markedly improved by the inclusion of even very limited experimental information. We have recently introduced an approach for the production of reliable topology models based on a combination of experimental determination of the location (cytoplasmic or periplasmic) of a protein's C terminus and topology prediction. Here, we show that determination of the location of a protein's C terminus, rather than some internal loop, is the best strategy for large-scale topology mapping studies. We further report experimentally based topology models for 31 Escherichia coli inner membrane proteins, using methodology suitable for genome-scale studies.
即使加入非常有限的实验信息，也能显著改进膜蛋白拓扑结构预测。我们最近引入了一种方法，通过结合蛋白质C端位置（胞质或周质）的实验测定和拓扑结构预测来生成可靠的拓扑模型。在这里，我们表明，确定蛋白质C端的位置，而不是某些内部环的位置，是大规模拓扑图谱研究的最佳策略。我们还使用适用于基因组规模研究的方法，报告了31种大肠杆菌内膜蛋白基于实验的拓扑模型。
