自体血小板凝胶的组成与特性

Composition and characteristics of an autologous thrombocyte gel.

作者信息

Altmeppen J, Hansen E, Bonnländer G L, Horch R E, Jeschke M G

机构信息

Department of Anaesthesia, University of Regensburg, Regensburg, Germany.

出版信息

J Surg Res. 2004 Apr;117(2):202-7. doi: 10.1016/j.jss.2003.10.019.

DOI:10.1016/j.jss.2003.10.019

PMID:15047124

Abstract

BACKGROUND

The purpose of the current study was to characterize and compare an autologous thrombocyte gel containing several blood components with a commercially available glue.

MATERIALS AND METHODS

Twenty-five volunteers had blood drawn, and lab values, characteristics of the platelet-enriched plasma (PRP), thrombocyte aggregation, electron microscopic examinations, and the breaking strength were determined and compared to a commercial glue.

RESULTS

Overall 65% of the total thrombocytes could be isolated from the volunteers and an enrichment of 300% with an autotransfusion device could be achieved. Thrombocyte aggregation as a marker for thrombocyte function decreased from 92% in patients to 71% in the PRP. The autologous glue demonstrated a significant reduced breaking strength (0,76 N/cm(3)) compared to the commercial glue (7.42 N/cm(2)), P < 0.05. The decrease in breaking strength could be correlated with the thrombocyte concentration, P < 0.05.

CONCLUSIONS

In the present study we have shown that an autologous platelet-enriched plasma cannot be used as a glue in the common sense to seal stitches or prosthesis. Platelet gels, however, have a high concentration of platelets that release the bioactive proteins and growth factors are necessary to initiate and accelerate tissue repair and enhance dermal and epidermal regeneration. To evaluate the possible clinical implication prospective, randomized studies should be performed to examine the effect of autologous plasma platelet-enriched plasma on wound healing.

摘要

背景

本研究的目的是对含有多种血液成分的自体血小板凝胶与一种市售胶水进行特性描述和比较。

材料与方法

抽取25名志愿者的血液，测定实验室值、富血小板血浆（PRP）的特性、血小板聚集情况、进行电子显微镜检查以及测定抗张强度，并与一种市售胶水进行比较。

结果

总体而言，可从志愿者体内分离出65%的血小板，使用自动输血装置可实现300%的富集。作为血小板功能标志物的血小板聚集率从患者体内的92%降至PRP中的71%。与市售胶水（7.42 N/cm²）相比，自体胶水的抗张强度显著降低（0.76 N/cm³），P < 0.05。抗张强度的降低与血小板浓度相关，P < 0.05。