Everts P A M, Hoffmann J, Weibrich G, Mahoney C B, Schönberger J P A M, van Zundert A, Knape J T A
Department of Extra Corporeal Blood Management, Catharina Hospital, Eindhoven, The Netherlands.
Transfus Med. 2006 Oct;16(5):363-8. doi: 10.1111/j.1365-3148.2006.00708.x.
Three commercial systems for whole blood separation were compared to obtain the buffy coat composed of platelet-rich plasma (BC-PRP) and leucocytes . These samples of the buffy coat were used to make a platelet gel (PG), which was used to measure platelet growth factor (PGF) release, to perform a white blood cell (WBC) count and to measure myeloperoxidase (MPO) release from WBCs. Aliquots of whole blood obtained from ten volunteers were distributed either to a blood cell separator (The Electa Cell-Separator, E-CS) or to a tabletop centrifuge (Gravitational Platelet Sequestration System, GPS) to prepare the BC-PRP. The third system combines the BC-PRP production by E-CS with a micro porous filter (Autologous Growth Factor filter, AGF) to enrich for the BC-PRP. Autologous thrombin was used to activate the BC-PRP and to prepare the PG and subsequently to degranulate the platelet concentrate. Platelet-derived growth factor-AB and transforming growth factor-beta1 were present in high levels after thrombin activation of the E-CS or GPS prepared samples. However, the AGF prepared samples released their growth factors before thrombin activation. The WBCs were significantly increased with each of the three systems. Contrary to the AGF, no leucocyte degranulation occurred with the E-CS or GPS prepared samples, based upon the low MPO concentrations in the BC-PRP. The three types of apparatus had different harvesting capacities for collecting the enriched platelets and the release of high concentrations of PGF. When the E-CS and GPS, but not the AGF, were used, low levels of MPO were maintained in the PG, which potentially contributes to antimicrobial properties of platelet gel at the site of application.
比较了三种用于全血分离的商业系统，以获取由富含血小板血浆（BC-PRP）和白细胞组成的血沉棕黄层。这些血沉棕黄层样本用于制备血小板凝胶（PG），该凝胶用于测量血小板生长因子（PGF）释放、进行白细胞（WBC）计数以及测量白细胞释放的髓过氧化物酶（MPO）。从十名志愿者采集的全血等分试样被分配到血细胞分离器（Electa细胞分离器，E-CS）或台式离心机（重力血小板分离系统，GPS）中，以制备BC-PRP。第三种系统将E-CS生产BC-PRP与微孔过滤器（自体生长因子过滤器，AGF）相结合，以富集BC-PRP。使用自体凝血酶激活BC-PRP并制备PG，随后使血小板浓缩物脱颗粒。在凝血酶激活E-CS或GPS制备的样本后，血小板衍生生长因子-AB和转化生长因子-β1含量很高。然而，AGF制备的样本在凝血酶激活前就释放了其生长因子。三种系统中的每一种都使白细胞显著增加。与AGF相反，基于BC-PRP中低MPO浓度，E-CS或GPS制备的样本未发生白细胞脱颗粒。这三种仪器在收集富集血小板和释放高浓度PGF方面具有不同的采集能力。当使用E-CS和GPS而非AGF时，PG中MPO水平较低，这可能有助于血小板凝胶在应用部位的抗菌特性。
