Sroka Jolanta, Kamiński Rafał, Michalik Marta, Madeja Zbigniew, Przestalski Stanisław, Korohoda Włodzimierz
Department of Cell Biology, Faculty of Biotechnology, Jagiellonian University, Gronostajowa 7, 30-387 Kraków, Poland.
Cell Mol Biol Lett. 2004;9(1):15-30.
The effect of triethyllead (TriEL) on the morphology and motile activity of Walker 256 carcinosarcoma cells was investigated. It was found that both 2 and 5 microM TriEL affected the cellular motility in a dose- and time-dependent manner. Initially, 2 microM TriEL caused the formation of blebs instead of lamellipodia at the front of some cells and stimulated the migration of Walker cells, but after 2 hours of 2 microM TriEL treatment, a reduction of cellular motility was observed. In the presence of 5 microM TriEL, Walker 256 carcinosarcoma cells rounded up, and their rate of movement was reduced. Moreover, the treatment of Walker carcinosarcoma cells with TriEL caused the disruption of microtubules and affected the F-actin distribution at both concentrations. At a concentration of 2 microM TriEL, the actin staining intensity was greatest in the tail of front-tail polarised blebbing cells and the actin layer was very thin at the leading edge. The control cells showed linear cortical F-actin distribution and somewhat less intense cytoplasmic staining at the same TriEL concentration. Cells treated with 5 microM TriEL showed an under-membrane pattern of actin distribution.
研究了三乙基铅（TriEL）对Walker 256癌肉瘤细胞形态和运动活性的影响。发现2 microM和5 microM的TriEL均以剂量和时间依赖性方式影响细胞运动。最初，2 microM的TriEL导致一些细胞前端形成泡状而非片状伪足，并刺激了Walker细胞的迁移，但在2 microM的TriEL处理2小时后，观察到细胞运动性降低。在5 microM的TriEL存在下，Walker 256癌肉瘤细胞变圆，其运动速率降低。此外，用TriEL处理Walker癌肉瘤细胞导致微管破坏，并在两种浓度下均影响F-肌动蛋白分布。在2 microM的TriEL浓度下，前端-尾部极化泡状细胞尾部的肌动蛋白染色强度最大，而前沿的肌动蛋白层非常薄。在相同的TriEL浓度下，对照细胞显示出线性的皮质F-肌动蛋白分布，细胞质染色强度略低。用5 microM的TriEL处理的细胞显示出肌动蛋白分布的膜下模式。
