钙和环核苷酸影响肿瘤坏死因子-α诱导的干细胞迁移。
Calcium and cyclic nucleotides affect TNF-alpha-induced stem cell migration.
作者信息
Kaplan Emel, Min Jiang-Yong, Ke Qingen, Chen Yu, Niethammer Marc, Rana Jamal S, Malek Sohail, Verheugt Freek W A, Morgan James P
机构信息
Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, MA, USA.
出版信息
Biochem Biophys Res Commun. 2009 May 1;382(2):241-6. doi: 10.1016/j.bbrc.2009.02.068. Epub 2009 Feb 20.
The purpose of this study was to study the effect of calcium, cyclic AMP (cAMP) and cyclic GMP (cGMP) on embryonic stem cell (ESC) motility during TNF-alpha-induced chemotaxis. ESCs were monitored using a chemotaxis chamber, with different concentrations of calcium or cAMP or cGMP added to the medium. Changes in intracellular calcium (Ca(2+)) were measured with the fluorescent dye fura-2/AM. We combined migratory parameters in a mathematical model and described it as "mobility". After adding calcium, a dose-dependant increase in cell speed was found. Cyclic AMP increased mobility as well as the Ca(2+). In contrast, adding dbcGMP resulted in a significant decrease in the mobility of the ESCs. During migration ESCs showed an increase in Ca(2+). Furthermore, TNF-alpha dramatically increased the movement as well as the directionality of ESCs. These results demonstrate that ESCs are highly motile and respond to different concentrations of calcium in a dose-related manner.
本研究的目的是探讨钙、环磷酸腺苷（cAMP）和环磷酸鸟苷（cGMP）在肿瘤坏死因子-α（TNF-α）诱导的趋化作用过程中对胚胎干细胞（ESC）运动性的影响。使用趋化室监测胚胎干细胞，向培养基中添加不同浓度的钙、cAMP或cGMP。用荧光染料fura-2/AM测量细胞内钙（Ca(2+)）的变化。我们将迁移参数整合到一个数学模型中，并将其描述为“迁移率”。添加钙后，发现细胞速度呈剂量依赖性增加。cAMP增加了迁移率以及Ca(2+)。相反，添加二丁酰环磷鸟苷（dbcGMP）导致胚胎干细胞的迁移率显著降低。在迁移过程中，胚胎干细胞的Ca(2+)增加。此外，TNF-α显著增加了胚胎干细胞的运动以及方向性。这些结果表明，胚胎干细胞具有高度的运动性，并以剂量相关的方式对不同浓度的钙作出反应。
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