Kobayashi Naoki, Takata Hiroshi, Yokota Shumpei, Takiguchi Masafumi
Division of Viral Immunology, Center for AIDS Research, Kumamoto University, Kumamoto, Japan.
Eur J Immunol. 2004 Dec;34(12):3370-8. doi: 10.1002/eji.200425587.
Multi-color flow cytometric analysis on human CD8(+) T cell subsets revealed that CXCR4 is predominantly expressed on CD8(+) T cells with the naive CD27(+)CD28(+)CD45RA(+) phenotype, and is down-regulated during differentiation into those with an effector phenotype. The down-regulation of CXCR4 expression during peripheral differentiation was supported by the fact that the expression of CXCR4 on CD8(+) T cells was negatively correlated with that of perforin. The analysis of CCR5, CCR7, and CXCR4 co-expression further showed that CD8(+) T cells expressing a high level of CXCR4 are CCR7(+)CCR5(-) naive or central memory subsets, and those expressing a low level of CXCR4 were included in the CCR7(-)CCR5(+/-) memory/effector and effector subsets. Epstein Barr virus-specific CD8(+) T cells, which mostly express the memory phenotype, expressed CXCR4, while human cytomegalovirus-specific CD8(+) T cells, which mostly express the effector phenotype, partially expressed this receptor, showing that the expression of CXCR4 is also down-regulated during differentiation of viral antigen-specific CD8(+) T cells. The classification of human CD8(+) T cells based on the expression of these chemokine receptors should prove useful for studies that clarify the differentiation of human CD8(+) T cells.
对人类CD8(+) T细胞亚群进行的多色流式细胞术分析显示，CXCR4主要在具有初始CD27(+)CD28(+)CD45RA(+)表型的CD8(+) T细胞上表达，并且在分化为效应器表型的细胞过程中表达下调。CD8(+) T细胞上CXCR4表达的下调在体外分化过程中得到了证实，即CXCR4在CD8(+) T细胞上的表达与穿孔素的表达呈负相关。对CCR5、CCR7和CXCR4共表达的分析进一步表明，表达高水平CXCR4的CD8(+) T细胞是CCR7(+)CCR5(-)初始或中央记忆亚群，而表达低水平CXCR4的细胞则包括在CCR7(-)CCR5(+/-)记忆/效应器和效应器亚群中。主要表达记忆表型的爱泼斯坦-巴尔病毒特异性CD8(+) T细胞表达CXCR4，而主要表达效应器表型的人巨细胞病毒特异性CD8(+) T细胞部分表达该受体，这表明在病毒抗原特异性CD8(+) T细胞的分化过程中CXCR4的表达也下调。基于这些趋化因子受体表达对人类CD8(+) T细胞进行分类，对于阐明人类CD8(+) T细胞分化的研究应是有用的。
