Clemmens John, Hess Henry, Doot Robert, Matzke Carolyn M, Bachand George D, Vogel Viola
Department of Bioengineering and Center for Nanotechnology, University of Washington, Box 351721, Seattle, WA 98195, USA.
Lab Chip. 2004 Apr;4(2):83-6. doi: 10.1039/b317059d. Epub 2004 Feb 27.
Nanotechnology promises to enhance the functionality and sensitivity of miniaturized analytical systems. For example, nanoscale transport systems, which are driven by molecular motors, permit the controlled movement of select cargo along predetermined paths. Such shuttle systems may enhance the detection efficiency of an analytical system or facilitate the controlled assembly of sophisticated nanostructures if transport can be coordinated through complex track networks. This study determines the feasibility of complex track networks using kinesin motor proteins to actively transport microtubule shuttles along micropatterned surfaces. In particular, we describe the performance of three basic structural motifs: (1) crossing junctions, (2) directional sorters, and (3) concentrators. We also designed track networks that successfully sort and collect microtubule shuttles, pointing the way towards lab-on-a-chip devices powered by active transport instead of pressure-driven or electroosmotic flow.
纳米技术有望增强小型分析系统的功能和灵敏度。例如，由分子马达驱动的纳米级运输系统允许选定的货物沿着预定路径进行受控移动。如果运输可以通过复杂的轨道网络进行协调，那么这种穿梭系统可能会提高分析系统的检测效率，或促进复杂纳米结构的受控组装。本研究确定了使用驱动蛋白马达蛋白沿着微图案表面主动运输微管穿梭体的复杂轨道网络的可行性。特别是，我们描述了三种基本结构基序的性能：（1）交叉连接点，（2）定向分选器，以及（3）集中器。我们还设计了能够成功分选和收集微管穿梭体的轨道网络，为基于主动运输而非压力驱动或电渗流的芯片实验室设备指明了方向。
