Cotter Robert J, Gardner Ben D, Iltchenko Sergei, English Robert D
Middle Atlantic Mass Spectrometry Laboratory, Johns Hopkins University School of Medicine, 725 North Wolfe Street, Baltimore, MD 21205, USA.
Anal Chem. 2004 Apr 1;76(7):1976-81. doi: 10.1021/ac0349431.
The unique focusing properties of the curved-field reflectron provide a simple solution to the problem of compensating for the broad range of energies of product ions produced postsource in a time-of-flight mass spectrometer. This has been shown previously for the technique known as postsource decay, but in this report we demonstrate its use for tandem time-of-flight mass spectrometry using a high-performance MALDI time-of-flight instrument modified by the addition of a collision chamber to enable the recording of mass-selected product ions formed by collision-induced dissociation (CID). In particular, the curved-field reflectron enables the use of the full 20-keV kinetic energy provided by the ion source extraction voltage as the collision energy in the laboratory frame and obviates the need to reaccelerate the product ions, using a second "source" or "lift" cell. Results are presented for the collision-induced dissociation of fullerenes over a range of collision gas pressures and precursor ion attenuation. In addition, CID tandem mass spectra are obtained for several peptides.
弯曲场反射器独特的聚焦特性为补偿飞行时间质谱仪源后产生的产物离子的宽能量范围问题提供了一个简单的解决方案。这在之前被称为源后衰变的技术中已经得到了证明，但在本报告中，我们展示了其在串联飞行时间质谱中的应用，该应用使用了一台高性能的基质辅助激光解吸电离飞行时间仪器，通过添加一个碰撞室进行了改进，以能够记录由碰撞诱导解离（CID）形成的质量选择产物离子。特别是，弯曲场反射器能够在实验室坐标系中使用离子源提取电压提供的全部20 keV动能作为碰撞能量，并且无需使用第二个“源”或“提升”单元对产物离子进行重新加速。给出了在一系列碰撞气体压力和前体离子衰减情况下富勒烯的碰撞诱导解离结果。此外，还获得了几种肽的CID串联质谱。
