采用纸片扩散法、肉汤微量稀释法和Etest法对曲霉属进行伏立康唑药敏试验的比较研究
A comparative study of the disc diffusion method with the broth microdilution and Etest methods for voriconazole susceptibility testing of Aspergillus spp.
作者信息
Serrano M C, Ramírez M, Morilla D, Valverde A, Chávez M, Espinel-Ingroff A, Claro R, Fernández A, Almeida C, Martín-Mazuelos E
机构信息
Servicio de Microbiología, Hospital Universitario de Valme, Ctra Cádiz s/n, Sevilla 41014, Spain.
出版信息
J Antimicrob Chemother. 2004 May;53(5):739-42. doi: 10.1093/jac/dkh172. Epub 2004 Mar 31.
OBJECTIVE
The activity of voriconazole against Aspergillus spp. (n = 77) was tested by the Etest, disc diffusion and the NCCLS M38-A methods.
METHODS
Four Rhizomucor spp. isolates were included to study the suitability of the three susceptibility testing methods to detect isolates resistant to voriconazole. The disc diffusion method performed on Mueller-Hinton agar (Difco) supplemented with 2% glucose and Methylene Blue (0.5 mg/L) gave zone diameters with very clear border edges.
RESULTS
The disc diffusion method showed excellent correlation with the Etest and the NCCLS methods.
CONCLUSION
On the basis of our results, it appears that the disc diffusion test is a useful method for testing the activity of voriconazole against Aspergillus spp.
目的
采用Etest法、纸片扩散法和NCCLS M38 - A法检测伏立康唑对曲霉属（n = 77）的活性。
方法
纳入4株根毛霉属分离株，以研究这三种药敏试验方法检测对伏立康唑耐药分离株的适用性。在补充有2%葡萄糖和亚甲蓝（0.5 mg/L）的穆勒-欣顿琼脂（Difco）上进行的纸片扩散法，其抑菌圈直径边界清晰。
结果
纸片扩散法与Etest法和NCCLS法显示出极好的相关性。
结论
根据我们的结果，纸片扩散试验似乎是检测伏立康唑对曲霉属活性的一种有用方法。
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