Espinel-Ingroff A, Canton E
Virginia Commonwealth University Medical Center, Richmond, USA.
J Clin Microbiol. 2008 May;46(5):1793-803. doi: 10.1128/JCM.01883-07. Epub 2008 Mar 12.
We compared the Neo-Sensitabs tablet assay to both reference M38-A broth microdilution and disk diffusion methods for testing the susceptibility of 183 filamentous isolates to amphotericin B, caspofungin, itraconazole, posaconazole, and voriconazole. Neo-Sensitabs and disk assay inhibition zone diameters, in millimeters, were obtained on nonsupplemented Mueller-Hinton agar at 16 to 48 h. The reproducibility of zone diameters (i.e., the percentage of replicate zone diameters that were within 2 standard deviations of the means), their correlation with either MICs or minimum effective concentrations (for caspofungin only), and the categorical agreement were similar between tablet and disk assays (93 to 100% [R, >0.70] and 79 to 96%, respectively) with four of the five agents. The exceptions were the results for posaconazole tablets (R, 0.686; disk, 0.757; 84% categorical agreement for tablet and 96% for disk). These data suggest the potential value of the Neo-Sensitabs assay for testing 5-microg caspofungin and 1-microg voriconazole posaconazole tablets against all mold isolates, 8-microg itraconazole and 5-microg tablets against all mold isolates except zygomycetes, and 10-microg amphotericin B tablets against zygomycete isolates only.
我们将Neo - Sensitabs平板试验与参考的M38 - A肉汤微量稀释法和纸片扩散法进行了比较，以检测183株丝状分离株对两性霉素B、卡泊芬净、伊曲康唑、泊沙康唑和伏立康唑的敏感性。在16至48小时内，在未添加补充剂的穆勒 - 欣顿琼脂上获得Neo - Sensitabs和纸片试验的抑菌圈直径（以毫米为单位）。平板试验和纸片试验之间，抑菌圈直径的重现性（即重复抑菌圈直径在均值的2个标准差范围内的百分比）、它们与最低抑菌浓度（MICs）或最低有效浓度（仅针对卡泊芬净）的相关性以及分类一致性相似（分别为93%至100%[R，>0.70]和79%至96%），涉及五种药物中的四种。例外情况是泊沙康唑片剂的结果（R，0.686；纸片法，0.757；片剂的分类一致性为84%，纸片法为96%）。这些数据表明，Neo - Sensitabs试验对于检测5μg卡泊芬净和1μg伏立康唑泊沙康唑片剂对所有霉菌分离株、8μg伊曲康唑和5μg片剂对除接合菌纲以外的所有霉菌分离株以及10μg两性霉素B片剂对仅接合菌纲分离株的潜在价值。
