Xiang Juan-juan, Nie Xin-min, Tang Jing-qun, Wang Yan-jin, Li Zheng, Gan Kai, Huang He, Xiong Wei, Li Xiao-ling, Li Gui-yuan
Cancer Research Institute, Xiangya School of Medicine, Central South University, Changsha 410078, China.
Zhonghua Zhong Liu Za Zhi. 2004 Feb;26(2):71-4.
To evaluate the feasibility of using iron oxide nanoparticles as gene vector and the effect of magnetic field on efficiency of transfection.
Iron oxide nanoparticles were prepared by alkaline precipitation of divalent and trivalent iron chloride. The surface of iron oxide nanoparticles was modified by self-assembled poly-L-lysine to form particle complexes (IONP-PLL). Transfection was determined by delivering reporter gene, PGL2-control encoding luciferase, to different cell lines using IONP-PLL as vector. The effect of magnetic field on efficiency of transfection was determined using Nd-Fe-B permanent magnet.
Foreign gene could be delivered to various cell lines by IONP-PLL and expressed with high efficiency, but the transfection efficiency and time course varied in the different cell lines studied. Magnetic field could enhance the efficiency of transfection by 5 - 10 fold.
IONP-PLL can be used as a novel non-viral gene vector in vitro, which offers a basis for gene delivery in vivo.
评估使用氧化铁纳米颗粒作为基因载体的可行性以及磁场对转染效率的影响。
通过二价和三价氯化铁的碱性沉淀制备氧化铁纳米颗粒。氧化铁纳米颗粒的表面通过自组装聚-L-赖氨酸进行修饰以形成颗粒复合物（IONP-PLL）。使用IONP-PLL作为载体，通过将编码荧光素酶的报告基因PGL2-对照递送至不同细胞系来测定转染情况。使用钕铁硼永磁体测定磁场对转染效率的影响。
外源基因可通过IONP-PLL递送至各种细胞系并高效表达，但在所研究的不同细胞系中转染效率和时间进程有所不同。磁场可使转染效率提高5至10倍。
IONP-PLL可作为一种新型的非病毒基因载体用于体外，这为体内基因递送提供了基础。
