Chen D, Tang Q, Xue W, Wang X
Key laboratory of Reproductive Medicine, Institute of Toxicology, Nanjing Medical University, Nanjing 210042, China.
West Indian Med J. 2010 Jun;59(3):300-5.
To evaluate the feasibility of using magnetic nanoparticles (MNPs) as gene vector and the effect of magnetic field on efficiency of transfection.
Magnetic nanoparticles were prepared by controlling some chemical reaction parameters through a partially reduction precipitation method with ferric chloride aqueous solution as precursor material. The surface of particles was modified by polyethyleneimine (PEI) agents. The appearance, the size distribution, structure and phase constitute of MNPs were characterized by Transmission electron microscope (TEM), X-ray diffraction (XRD); the potential of absorbing DNA of MNPs was analysed by electrophoresis. Transfection was determined by delivering reporter gene, PGL2-control encoding luciferase, to different cell lines using MNPs-PLL as vector. The effect of magnetic field on the efficiency of transfection was determined using Nd-Fe-B permanent magnet.
Foreign gene could be delivered to various cell lines by MNPs-PLL and expressed with high efficiency but the transfection efficiency and time course varied in the different cell lines studied. Magnetic field could enhance the efficiency of transfection by 5-10 fold.
MNPs- PLL can be used as a novel non-viral gene vector in vitro, which offers a basis for gene delivery in vivo.
评估使用磁性纳米颗粒（MNPs）作为基因载体的可行性以及磁场对转染效率的影响。
以氯化铁水溶液为前驱体材料，通过控制部分化学反应参数，采用部分还原沉淀法制备磁性纳米颗粒。颗粒表面用聚乙烯亚胺（PEI）试剂进行修饰。用透射电子显微镜（TEM）、X射线衍射（XRD）对MNPs的外观、尺寸分布、结构和相组成进行表征；通过电泳分析MNPs吸附DNA的能力。以MNPs-PLL为载体，将编码荧光素酶的报告基因PGL2-control导入不同细胞系来测定转染情况。使用钕铁硼永磁体测定磁场对转染效率的影响。
MNPs-PLL可将外源基因导入多种细胞系并高效表达，但在所研究的不同细胞系中转染效率和时间进程有所不同。磁场可使转染效率提高5至10倍。
MNPs-PLL可作为一种新型的非病毒基因载体用于体外实验，为体内基因递送提供了依据。
