Shen Qin, Goderie Susan K, Jin Li, Karanth Nithin, Sun Yu, Abramova Natalia, Vincent Peter, Pumiglia Kevin, Temple Sally
Center for Neuropharmacology and Neuroscience, Albany Medical College, Albany, NY 12208, USA.
Science. 2004 May 28;304(5675):1338-40. doi: 10.1126/science.1095505. Epub 2004 Apr 1.
Neural stem cells are reported to lie in a vascular niche, but there is no direct evidence for a functional relationship between the stem cells and blood vessel component cells. We show that endothelial cells but not vascular smooth muscle cells release soluble factors that stimulate the self-renewal of neural stem cells, inhibit their differentiation, and enhance their neuron production. Both embryonic and adult neural stem cells respond, allowing extensive production of both projection neuron and interneuron types in vitro. Endothelial coculture stimulates neuroepithelial cell contact, activating Notch and Hes 1 to promote self-renewal. These findings identify endothelial cells as a critical component of the neural stem cell niche.
据报道，神经干细胞位于血管微环境中，但干细胞与血管组成细胞之间功能关系的直接证据尚不明确。我们发现，内皮细胞而非血管平滑肌细胞能释放可刺激神经干细胞自我更新、抑制其分化并增强其神经元生成的可溶性因子。胚胎期和成年期神经干细胞均对此有反应，从而在体外大量生成投射神经元和中间神经元类型。内皮细胞共培养可刺激神经上皮细胞接触，激活Notch和Hes 1以促进自我更新。这些发现确定内皮细胞是神经干细胞微环境的关键组成部分。
