Chachkhiani M, Dabert P, Abzianidze T, Partskhaladze G, Tsiklauri L, Dudauri T, Godon J J
High Technology National Center, 21 Kavtaradze street, Tbilisi, Georgia.
Bioresour Technol. 2004 Jul;93(3):227-32. doi: 10.1016/j.biortech.2003.11.005.
A laboratory-scale continuously stirred anaerobic thermophilic batch digester was inoculated with cattle manure. Bacterial and archaeal communities, as well as digester performances, were analysed during reactor start-up for about 20 days. Polymerase chain reaction-single strand conformation polymorphism (PCR-SSCP) was used for overall detection and for study of the dynamics of microbial populations. Dominant bacteria and archaea 16S rDNAs were sequenced from the sample on day 12. Ten bacteria and 3 archaea OTUs (operational taxonomic units) were identified from the 52 clones sequenced. Sequences corresponding to the dominant bacterial SSCP peak were phylogenetically close to the 16S rDNA sequence of Bacillus thermoterrestris, whereas sequences corresponding to the two dominant archaeal SSCP peaks were phylogenetically close to the 16S rDNA sequence of Methanoculleus thermophilicus and Methanosarcina thermophila.
一个实验室规模的连续搅拌厌氧嗜热间歇式消化器接种了牛粪。在反应器启动约20天的过程中，分析了细菌和古菌群落以及消化器性能。聚合酶链反应-单链构象多态性（PCR-SSCP）用于整体检测和微生物种群动态研究。在第12天从样品中对优势细菌和古菌的16S rDNA进行了测序。从测序的52个克隆中鉴定出10个细菌和3个古菌OTU（操作分类单元）。与优势细菌SSCP峰相对应的序列在系统发育上与嗜热栖热芽孢杆菌的16S rDNA序列接近，而与两个优势古菌SSCP峰相对应的序列在系统发育上与嗜热甲烷袋状菌和嗜热甲烷八叠球菌的16S rDNA序列接近。
