Department of Biological and Environmental Science, University of Jyväskylä, P.O. Box 35 (NSC), 40014 University of Jyväskylä, Finland.
Biodegradation. 2010 Feb;21(1):135-46. doi: 10.1007/s10532-009-9288-5. Epub 2009 Jul 30.
The impacts of feeding ratio and loading rate on the microbial community during co-digestion of grass silage with cow manure in an anaerobic laboratory continuously stirred tank reactor were investigated by 16S rRNA gene-based fingerprints. The microbial community remained stable when the reactor was fed with cow manure alone and with up to 20% of grass silage in feedstock at an organic loading rate (OLR) of 2 kg VS m(-3) day(-1). Large changes in the bacterial community were observed when the loading ratio of grass was increased to 40%, while there was little change in the archaeal community. During the increase in OLR from 2 to 4 kg VS m(-3) day(-1) the bacterial community structure showed few differences, whereas Archaea was undetectable. Sequencing of the major DGGE bands indicated that the phylum Bacteriodetes predominated in the bacterial community. Two unclassified bacteria with high abundance survived throughout the operation of the reactor.
采用基于 16S rRNA 基因指纹图谱的方法研究了在厌氧实验室连续搅拌罐式反应器中，草青贮与牛粪共消化时，进料比和进料速率对微生物群落的影响。当反应器仅以牛粪为进料且进料中草青贮的比例高达 20%，在有机负荷率（OLR）为 2kgVS/m3·d 时，微生物群落保持稳定。当草的进料比例增加到 40%时，细菌群落发生了很大变化，而古菌群落变化很小。当 OLR 从 2kgVS/m3·d 增加到 4kgVS/m3·d 时，细菌群落结构几乎没有差异，而古菌则无法检测到。主要 DGGE 条带的测序表明，拟杆菌门在细菌群落中占优势。两种丰度较高的未分类细菌在整个反应器的运行过程中存活下来。
