Ceol Craig J, Horvitz H R
Howard Hughes Medical Institute, Department of Biology, 68-425, Massachusetts Institute of Technology, Cambridge, MA 02139, USA.
Dev Cell. 2004 Apr;6(4):563-76. doi: 10.1016/s1534-5807(04)00065-6.
The class A and class B synMuv genes are functionally redundant negative regulators of a Ras signaling pathway that induces C. elegans vulval development. A number of class B synMuv genes encode components of an Rb and histone deacetylase complex that likely acts to repress transcription of genes required for vulval induction. We discovered a new class of synMuv genes that acts redundantly with both the A and B classes of genes in vulval cell-fate determination. These new class C synMuv genes encode TRRAP, MYST family histone acetyltransferase, and Enhancer of Polycomb homologs, which form a putative C. elegans Tip60/NuA4-like histone acetyltransferase complex. A fourth gene with partial class C synMuv properties encodes a homolog of the mammalian SWI/SNF family ATPase p400. Our findings indicate that the coordinated action of two chromatin-modifying complexes, one with histone deacetylase and the other with histone acetyltransferase activity, is important in regulating Ras signaling and specifying cell fates during C. elegans development.
A类和B类synMuv基因是Ras信号通路的功能冗余负调控因子，该信号通路诱导秀丽隐杆线虫的外阴发育。许多B类synMuv基因编码Rb和组蛋白脱乙酰酶复合物的组分，该复合物可能作用于抑制外阴诱导所需基因的转录。我们发现了一类新的synMuv基因，其在决定外阴细胞命运方面与A类和B类基因发挥冗余作用。这些新的C类synMuv基因编码TRRAP、MYST家族组蛋白乙酰转移酶和多梳蛋白同源增强子，它们形成一个假定的秀丽隐杆线虫Tip60/NuA4样组蛋白乙酰转移酶复合物。第四个具有部分C类synMuv特性的基因编码哺乳动物SWI/SNF家族ATP酶p400的一个同源物。我们的研究结果表明，两种染色质修饰复合物的协同作用在调控秀丽隐杆线虫发育过程中的Ras信号和确定细胞命运方面很重要，其中一种复合物具有组蛋白脱乙酰酶活性，另一种具有组蛋白乙酰转移酶活性。
