Ceol Craig J, Stegmeier Frank, Harrison Melissa M, Horvitz H Robert
Department of Biology, Howard Hughes Medical Institute, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA.
Genetics. 2006 Jun;173(2):709-26. doi: 10.1534/genetics.106.056465. Epub 2006 Apr 19.
The synthetic multivulva (synMuv) genes negatively regulate Ras-mediated vulval induction in the nematode Caenorhabditis elegans. The synMuv genes define three classes, A, B, and C, such that double mutants carrying mutations in genes of any two classes are multivulva. The class B synMuv genes include lin-35, a homolog of the retinoblastoma (Rb) tumor suppressor gene, as well as homologs of genes that function with Rb in transcriptional regulation. We screened for additional synMuv mutations using a strategy different from that of previous synMuv genetic screens. Some of the mutations we recovered affect new synMuv genes. We present criteria for assigning synMuv mutations into different genetic classes. We also describe the molecular characterization of the class B synMuv gene lin-65.
合成多外阴（synMuv）基因对线虫秀丽隐杆线虫中Ras介导的外阴诱导起负调控作用。synMuv基因分为A、B和C三类，因此在任意两类基因中携带突变的双突变体都是多外阴的。B类synMuv基因包括视网膜母细胞瘤（Rb）肿瘤抑制基因的同源物lin-35，以及在转录调控中与Rb起作用的基因的同源物。我们采用了一种不同于以往synMuv基因筛选的策略来筛选额外的synMuv突变。我们获得的一些突变影响新的synMuv基因。我们提出了将synMuv突变分配到不同遗传类别的标准。我们还描述了B类synMuv基因lin-65的分子特征。
