Rothberg Brad S
Department of Physiology, University of Texas Health Science Center, San Antonio, TX 78229, USA.
Sci STKE. 2004 Mar 30;2004(227):pe16. doi: 10.1126/stke.2272004pe16.
Maxi-K channel gating is modulated by both membrane voltage and cytoplasmic Ca2+. Recent work has led to a quantitative understanding of this dual regulatory mechanism, derived from classical allosteric principles, in terms of the energetic coupling among membrane voltage, Ca2+ binding, and opening of the channel's gate. Our new understanding of the gating pathway can be applied to the interpretation of mutagenesis experiments and may serve as an example in the analysis of other dually regulated signaling molecules.
大电导钙激活钾通道（Maxi-K通道）的门控受膜电压和胞质Ca2+的双重调节。最近的研究工作已从经典的别构原理出发，在膜电压、Ca2+结合以及通道门开放之间的能量耦合方面，对这种双重调节机制有了定量的理解。我们对门控途径的新认识可应用于诱变实验的解释，并且可能成为分析其他双重调节信号分子的一个范例。
