Corrado Marcia, Rodrigues Katia F
Department of Mycology, Oswaldo Cruz Institute, FIOCRUZ, P.O. Box 926, 21045-900, Rio de Janeiro, RJ, Brazil.
J Basic Microbiol. 2004;44(2):157-60. doi: 10.1002/jobm.200310341.
Crude extract of cultures of 13 fungal strains identified as Phomopsis sp. and isolated as endophytes from the leaves of Aspidosperma tomentosum and twigs of Spondias mombin were examined for their antibacterial and antifungal activities. The screening was conducted using the bioautographic TLC agar-overlay technique against bacteria (E. coli, P. aeruginosa, S. aureus), yeast (C. albicans, S. cerevisiae), and readily adapted for use with filamentous fungi (A. niger, F. oxysporum). Three of the 13 extracts effectively inhibited the growth of all test-organisms, indicating that they may represent a potential for pharmaceutical and/or agricultural applications and are worthy of further study.
对13株被鉴定为拟茎点霉属（Phomopsis sp.）的真菌菌株的培养物粗提物进行了研究，这些菌株作为内生菌从绒毛山辣椒（Aspidosperma tomentosum）的叶片和腰果树（Spondias mombin）的嫩枝中分离得到，检测了它们的抗菌和抗真菌活性。使用生物自显影薄层色谱琼脂覆盖技术对细菌（大肠杆菌、铜绿假单胞菌、金黄色葡萄球菌）、酵母（白色念珠菌、酿酒酵母）以及易于用于丝状真菌（黑曲霉、尖孢镰刀菌）进行筛选。13种提取物中的3种有效抑制了所有测试微生物的生长，表明它们可能具有制药和/或农业应用潜力，值得进一步研究。
