Defence Research Laboratory, Tezpur, 784001 Assam India.
Bilaspur Vishwavidyalaya, Bilaspur, 495001 Chhattisgarh India ; Department of Life Science and Bioinformatics, Assam University, Silchar, 788011 India.
Indian J Microbiol. 2014 Sep;54(3):302-9. doi: 10.1007/s12088-014-0458-8. Epub 2014 Feb 19.
An endophytic fungus isolated from Camellia sinensis, Assam, Northeastern India was identified as Colletotrichum gloeosporioides on the basis of morphological characteristics and rDNA ITS analysis. This endophytic fungus was evaluated for growth inhibition against tea pathogens Pestalotiopsis theae and Colletotrichum camelliae. One isolate of C. gloeosporioides showed strong antagonistic activity against Pestalotiopsis theae (64 %) and moderate activity against C. camelliae (37 %). Fifty percent cell-free culture filtrate from 5-day-old cultures showed highest antagonistic activity against both the pathogens although the inhibition percent was less as compared to dual culture. In the experiment of volatile compounds none of the isolates of C. gloeosporioides strains showed visible inhibition against P. theae and C. camelliae. The activity of extracellular hydrolytic enzymes chitinase and protease was also high in this culture fluid and measured 10 and 4.3 IU/μl, respectively.
从印度东北部阿萨姆邦的茶树中分离出的一种内生真菌，根据形态特征和 rDNA ITS 分析，被鉴定为胶孢炭疽菌。该内生真菌对茶病原菌拟盘多毛孢和炭疽菌的生长抑制作用进行了评价。其中一个胶孢炭疽菌分离株对拟盘多毛孢（64%）表现出强烈的拮抗活性，对炭疽菌（37%）表现出中等活性。在 5 天龄的培养物中，无细胞培养滤液的 50%对两种病原菌均表现出最高的拮抗活性，尽管抑制百分比较双培养物低。在挥发性化合物的实验中，没有一种胶孢炭疽菌分离株对拟盘多毛孢和炭疽菌表现出明显的抑制作用。该培养液中胞外水解酶几丁质酶和蛋白酶的活性也很高，分别为 10 和 4.3 IU/μl。
