用于检测耐万古霉素肠球菌物种中vanA和vanB基因的Velogene基因组检测法的评估。

Evaluation of the Velogene genomic assay for detection of vanA and vanB genes in vancomycin-resistant Enterococcus species.

作者信息

Appleman Maria D, Citron Diane M, Kwok Richard

机构信息

Department of Pathology, Keck School of Medicine, University of Southern California, Los Angeles County, California 90033, USA.

出版信息

J Clin Microbiol. 2004 Apr;42(4):1751-2. doi: 10.1128/JCM.42.4.1751-1752.2004.

DOI:10.1128/JCM.42.4.1751-1752.2004

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC387590/

Abstract

The Velogene (VEL) genomic assay is a qualitative DNA probe for vanA and vanB in enterococci. 150 clinical isolates were tested with the VEL assay and characterized by pigment production, catalase levels, motility, growth in 6 micro g of vancomycin/ml, vancomycin and teicoplanin susceptibility, API 20S assay, and genotyping by multiplex PCR. The VEL assay identified all enterococcal strains with vanA and vanB genes.

摘要

Velogene（VEL）基因组检测法是一种用于检测肠球菌中vanA和vanB基因的定性DNA探针。使用VEL检测法对150株临床分离株进行检测，并通过色素产生、过氧化氢酶水平、运动性、在6μg/ml万古霉素中的生长情况、万古霉素和替考拉宁敏感性、API 20S检测法以及多重PCR基因分型进行特征分析。VEL检测法鉴定出了所有携带vanA和vanB基因的肠球菌菌株。

相似文献

1

Evaluation of the Velogene genomic assay for detection of vanA and vanB genes in vancomycin-resistant Enterococcus species.用于检测耐万古霉素肠球菌物种中vanA和vanB基因的Velogene基因组检测法的评估。

J Clin Microbiol. 2004 Apr;42(4):1751-2. doi: 10.1128/JCM.42.4.1751-1752.2004.

2

Emergence of vanA Genotype Vancomycin-Resistant Enterococci with Low or Moderate Levels of Teicoplanin Resistance in Korea.韩国出现具有低或中度替考拉宁耐药水平的vanA基因型耐万古霉素肠球菌。

J Clin Microbiol. 2004 Apr;42(4):1785-6. doi: 10.1128/JCM.42.4.1785-1786.2004.

3

Emergence of vancomycin-resistant enterococci in Australia: phenotypic and genotypic characteristics of isolates.澳大利亚耐万古霉素肠球菌的出现：分离株的表型和基因型特征

J Clin Microbiol. 1998 Aug;36(8):2187-90. doi: 10.1128/JCM.36.8.2187-2190.1998.

4

Development of the Specific and Random Amplification (SARA)-PCR for both species identification of enterococci and detection of the vanA gene.用于肠球菌菌种鉴定及vanA基因检测的特异性随机扩增（SARA）-PCR技术的开发。

J Microbiol Methods. 2001 Jan;43(3):233-9. doi: 10.1016/s0167-7012(00)00225-6.

5

Multiplex PCR detection of vanA, vanB, vanC-1, and vanC-2/3 genes in enterococci.肠球菌中vanA、vanB、vanC-1和vanC-2/3基因的多重聚合酶链反应检测

J Clin Microbiol. 1997 Mar;35(3):703-7. doi: 10.1128/jcm.35.3.703-707.1997.

6

Lipophilic teicoplanin pseudoaglycon derivatives are active against vancomycin- and teicoplanin-resistant enterococci.亲脂性替考拉宁假糖苷配基衍生物对万古霉素和替考拉宁耐药肠球菌具有活性。

J Antibiot (Tokyo). 2017 May;70(5):664-670. doi: 10.1038/ja.2017.2. Epub 2017 Feb 1.

7

First description in Europe of the emergence of Enterococcus faecium ST117 carrying both vanA and vanB genes, isolated in Greece.首次在欧洲描述携带 vanA 和 vanB 基因的屎肠球菌 ST117 的出现，该菌在希腊被分离。

J Glob Antimicrob Resist. 2017 Dec;11:68-70. doi: 10.1016/j.jgar.2017.07.010. Epub 2017 Jul 25.

8

[Phenotypic and genotypic traits of vancomycin-resistant enterococci in a public hospital: the first vanB-positive Enterococcus faecium isolates].[一家公立医院中耐万古霉素肠球菌的表型和基因型特征：首例vanB阳性粪肠球菌分离株]

Mikrobiyol Bul. 2012 Apr;46(2):276-82.

9

Evaluation of vancomycin resistance 3 multiplexed PCR assay for detection of vancomycin-resistant enterococci from rectal swabs.用于从直肠拭子中检测耐万古霉素肠球菌的万古霉素耐药性3重多重聚合酶链反应检测法的评估

Ann Lab Med. 2013 Sep;33(5):326-30. doi: 10.3343/alm.2013.33.5.326. Epub 2013 Aug 8.

10

Real-time PCR for the rapid detection of vanA, vanB and vanM genes.实时 PCR 快速检测 vanA、vanB 和 vanM 基因。

J Microbiol Immunol Infect. 2020 Oct;53(5):746-750. doi: 10.1016/j.jmii.2019.02.002. Epub 2019 Mar 13.

本文引用的文献

1

Emergence of vancomycin-resistant enterococci.耐万古霉素肠球菌的出现。

Emerg Infect Dis. 2001 Mar-Apr;7(2):183-7. doi: 10.3201/eid0702.010205.

2

Insights into the epidemiology and control of infection with vancomycin-resistant enterococci.耐万古霉素肠球菌感染的流行病学及控制研究进展

Clin Infect Dis. 2000 Oct;31(4):1058-65. doi: 10.1086/318126. Epub 2000 Oct 25.

3

Vancomycin-resistant enterococci.耐万古霉素肠球菌

Clin Microbiol Rev. 2000 Oct;13(4):686-707. doi: 10.1128/CMR.13.4.686.

4

Detection of vancomycin resistant genes vanA and vanB by cycling probe technology.运用循环探针技术检测万古霉素耐药基因vanA和vanB

Mol Cell Probes. 1999 Jun;13(3):223-31. doi: 10.1006/mcpr.1999.0242.

5

Cycling probe technology with RNase H attached to an oligonucleotide.将核糖核酸酶H附着于寡核苷酸的循环探针技术。

Biotechniques. 1996 Feb;20(2):240-8. doi: 10.2144/96202rr01.

6

Plasmid-mediated resistance to vancomycin and teicoplanin in Enterococcus faecium.粪肠球菌中质粒介导的对万古霉素和替考拉宁的耐药性

N Engl J Med. 1988 Jul 21;319(3):157-61. doi: 10.1056/NEJM198807213190307.

文献AI研究员

20分钟写一篇综述，助力文献阅读效率提升50倍。

用中文搜PubMed

大模型驱动的PubMed中文搜索引擎

文档翻译

学术文献翻译模型，支持多种主流文档格式。