Yoshii Satoru, Oka Masanori, Shima Mitsuhiro, Taniguchi Ataru, Akagi Masao
Institute of Biomedical Engineering, Kansai Denryoku Hospital, Imaichi 2-7-14, Asahi-ku, Osaka, 535-0011 Japan.
J Biomed Mater Res A. 2003 Nov 1;67(2):467-74. doi: 10.1002/jbm.a.10103.
This article describes a 30-mm regeneration of severed peripheral nerve axons along collagen filaments. Two thousand or 4000 31-mm-long collagen filaments were grafted to bridge a 30-mm defect of the rat sciatic nerve. A collagen tube was grafted as a control. The mean number and mean fiber diameter of regenerated myelinated axons were 330 +/- 227 and 2.7 +/- 0.9 microm in the distal end of the 2000 collagen-filaments nerve guide, and 564 +/- 275 and 2.5 +/- 1.1 microm in the distal end of the 4000 collagen-filaments nerve guide at 12 weeks postoperatively, whereas in the distal end of the collagen tube, no regenerated axon was found. These results suggest that the collagen filaments guide axons of the rat's sciatic nerve to regenerate for 30 mm and act as a scaffold for axonal regeneration. Thirty-millimeter nerve regeneration of a 1-mm-diameter rat sciatic nerve by an artificial nerve guarantees a clinical application of the implant which should be very important for patients and surgeons.
本文描述了切断的周围神经轴突沿胶原丝的30毫米再生。将2000根或4000根31毫米长的胶原丝移植到大鼠坐骨神经30毫米的缺损处，以桥接缺损。作为对照，移植了一个胶原管。术后12周，在2000根胶原丝神经导管远端，再生有髓轴突的平均数量和平均纤维直径分别为330±227和2.7±0.9微米；在4000根胶原丝神经导管远端，分别为564±275和2.5±1.1微米，而在胶原管远端未发现再生轴突。这些结果表明，胶原丝可引导大鼠坐骨神经轴突再生30毫米，并作为轴突再生的支架。通过人工神经实现1毫米直径大鼠坐骨神经30毫米的神经再生，保证了该植入物的临床应用，这对患者和外科医生来说非常重要。
