Fuenzalida-Valdivia Isabel, Herrera-Vásquez Ariel, Gangas María Victoria, Sáez-Vásquez Julio, Álvarez José Miguel, Meneses Claudio, Blanco-Herrera Francisca
Centro de Biotecnología Vegetal, Facultad de Ciencias de la Vida, Universidad Andrés Bello, Santiago, Chile.
ANID-Millennium Science Initiative Program-Millennium Nucleus for the Development of Super Adaptable Plants (MN-SAP), Santiago, Chile.
Mol Plant Pathol. 2024 Dec;25(12):e70044. doi: 10.1111/mpp.70044.
In Arabidopsis thaliana, the transcription factors WRKY7, WRKY11 and WRKY17 act as negative defence regulators against Pseudomonas syringae pv. tomato (Pst) DC3000. However, their coordinated regulation of gene expression has yet to be fully explored. In this study, we conducted a transcriptomic analysis on the triple mutant wrky7/11/17 in response to Pst DC3000 at 0, 3 and 24 h post-inoculation (hpi). Our results suggest that at early infection stages (0 and 3 hpi), WRKY7, WRKY11 and WRKY17 significantly repress a group of genes involved in signal perception and transduction, including receptor-like kinases. Furthermore, at later stages of interaction (24 hpi), these transcription factors induce genes related to the biosynthesis and signalling of the jasmonic acid (JA) pathway. Further infection experiments with Pst DC3000 in plants treated with methyl jasmonate (a JA analogue) and infections with Botrytis cinerea, a pathogen against which JA-mediated responses are crucial for effective defence, support this proposal. Moreover, we analysed the role of WRKY7, WRKY11 and WRKY17 in alternative splicing regulation. A comparison between differentially expressed (DEG) and spliced (DAS) genes revealed that over 80% of DAS events do not occur in conjunction with overall changes in gene expression. Alternative splicing events were found in genes with functions in splicing and the JA pathway, such as ALY4, PRP40A, JAZ3 and JAZ10. These results suggest that WRKY7, WRKY11 and WRKY17 can also participate in this layer of gene expression regulation to modulate immunity negatively.
在拟南芥中，转录因子WRKY7、WRKY11和WRKY17作为针对丁香假单胞菌番茄致病变种（Pst）DC3000的负向防御调节因子。然而，它们对基因表达的协同调控尚未得到充分研究。在本研究中，我们对接种Pst DC3000后0、3和24小时（hpi）的三突变体wrky7/11/17进行了转录组分析。我们的结果表明，在感染早期阶段（0和3 hpi），WRKY7、WRKY11和WRKY17显著抑制一组参与信号感知和转导的基因，包括类受体激酶。此外，在相互作用的后期阶段（24 hpi），这些转录因子诱导与茉莉酸（JA）途径的生物合成和信号传导相关的基因。用茉莉酸甲酯（一种JA类似物）处理的植物中进一步进行的Pst DC3000感染实验以及对灰葡萄孢的感染实验（针对该病原体，JA介导的反应对于有效防御至关重要）支持了这一观点。此外，我们分析了WRKY7、WRKY11和WRKY17在可变剪接调控中的作用。差异表达（DEG）基因和剪接（DAS）基因之间的比较表明，超过80%的DAS事件并非与基因表达的整体变化同时发生。在具有剪接和JA途径功能的基因中发现了可变剪接事件，如ALY4、PRP40A、JAZ3和JAZ10。这些结果表明，WRKY7、WRKY11和WRKY17也可以参与这一层基因表达调控，以负向调节免疫。
