Watanabe Naoto, Kanegane Hirokazu, Kinuya Seigo, Shuke Noriyuki, Yokoyama Kunihiko, Kato Hiroshi, Tomizawa Gakuto, Shimizu Masashi, Funada Hisashi, Seto Hikaru
Department of Radiology, Toyama Medical and Pharmaceutical University, Toyama, Japan.
J Nucl Med. 2004 Apr;45(4):608-11.
We investigated cytologic radiation damage in thyroid cancer after (131)I therapy using micronucleus assay (MNA) of B lymphocytes exclusively, as opposed to our previous study in which MNA of all lymphocyte subsets was used.
We studied 22 thyroid cancer patients treated with 3.7 GBq of (131)I. Peripheral lymphocytes were harvested, and B lymphocytes were isolated by an immunomagnetic method and assayed for the frequency of micronuclei.
The frequency of micronuclei among B cells after (131)I therapy was significantly increased relative to that in untreated control subjects, and the (131)I-induced increase in micronuclei frequency among B cells was significantly greater than that among all lymphocytes.
Compared with the MNA of all lymphocytes, the MNA among specifically B cells may more sensitively detect cytologic radiation damage associated with (131)I therapy of thyroid cancer.
我们专门使用B淋巴细胞的微核试验（MNA）研究了（131）I治疗后甲状腺癌的细胞辐射损伤，这与我们之前使用所有淋巴细胞亚群的MNA的研究相反。
我们研究了22例接受3.7GBq（131）I治疗的甲状腺癌患者。采集外周血淋巴细胞，通过免疫磁珠法分离B淋巴细胞，并检测微核频率。
与未治疗的对照受试者相比，（131）I治疗后B细胞中的微核频率显著增加，并且（131）I诱导的B细胞微核频率增加显著大于所有淋巴细胞中的增加。
与所有淋巴细胞的MNA相比，特定B细胞中的MNA可能更敏感地检测与甲状腺癌（131）I治疗相关的细胞辐射损伤。
