Li Li, Hui James Hoi Po, Goh James Cho Hong, Chen Fen, Lee Eng Hin
Department of Orthopaedic Surgery, National University of Singapore, Singapore.
J Pediatr Orthop. 2004 Mar-Apr;24(2):205-10. doi: 10.1097/00004694-200403000-00014.
To investigate the feasibility of using chitin, a natural polymer, as a scaffold to repair large growth plate defects (50% of physis) in immature rabbits, mesenchymal stem cells (MSCs) were harvested from periosteum. The compatibilities of chitin with the MSCs were investigated in vitro using immunohistochemistry and fluorescence confocal microscopy. The results showed high compatibilities of chitin. An experimental model of growth arrest was created by excising the medial half of the proximal growth plate of the tibia in 6-week-old New Zealand White rabbits. The physeal defect after excision of the bony bridge was transplanted either with no interposition (group 1), chitin alone (group 2), or chitin with MSCs (group 3). In groups 2 and 3, both angulatory deformities and length discrepancies of the tibia were corrected. The differences between group 3 and group 1 were greater than those between group 2 and group 1.
为研究使用天然聚合物几丁质作为支架修复未成熟兔大的生长板缺损（占生长板的50%）的可行性，从骨膜中采集间充质干细胞（MSCs）。使用免疫组织化学和荧光共聚焦显微镜在体外研究几丁质与MSCs的相容性。结果显示几丁质具有高度相容性。通过切除6周龄新西兰白兔胫骨近端生长板内侧一半建立生长停滞实验模型。切除骨桥后的生长板缺损处，分别不进行植入（第1组）、单独植入几丁质（第2组）或植入几丁质与MSCs（第3组）。在第2组和第3组中，胫骨的成角畸形和长度差异均得到纠正。第3组与第1组之间的差异大于第2组与第1组之间的差异。
