Jiang Rong-cai, Pu Pei-yu, Shen Chang-hong, Yu Shiz-hu, Jiao Bao-hua, Wang Hu, Wang Chun-yan, Kang Chun-sheng
Department of Neurosurgery, Tianjin Medical University General Hospital, Tianjin, 300052 PR China.
Zhonghua Yi Xue Yi Chuan Xue Za Zhi. 2004 Apr;21(2):179-81.
To explore the molecular genesis of medulloblastomas with cDNA array.
Four samples of medulloblastomas and 1 sample of normal brain tissue were collected freshly. After total RNA extraction, the (32)P targeted cDNA probes were converted and then hybridized with Atlas Human Cancer Array 1.2. The gene expression profiles were acquired through autoradiography. The discrepancy between the tumor and the normal brain tissue was analyzed with Atlas Image 1.01a.
In comparison with the genes in the normal brain tissue, 6 down-regulated and 35 up-regulated genes in the medulloblastomas were revealed by means of the microarrays and autoradiography, and were verified by reverse transcriptase-PCR. The regulatory trends of most differential expression genes were in compliance with the biological features of this tumor.
Medulloblastomas are diseases involving multiple genes with some molecular pathological mechanisms different from the astrocytic gliomas. There are complex interrelationships between these genes, which need to be further researched.
利用cDNA芯片探讨髓母细胞瘤的分子发生机制。
新鲜采集4例髓母细胞瘤样本和1例正常脑组织样本。提取总RNA后，将（32）P标记的cDNA探针进行转换，然后与Atlas Human Cancer Array 1.2杂交。通过放射自显影获得基因表达谱。用Atlas Image 1.01a分析肿瘤组织与正常脑组织之间的差异。
通过微阵列和放射自显影技术，与正常脑组织中的基因相比，在髓母细胞瘤中发现了6个下调基因和35个上调基因，并通过逆转录-聚合酶链反应进行了验证。大多数差异表达基因的调控趋势与该肿瘤的生物学特征相符。
髓母细胞瘤是涉及多个基因的疾病，其分子病理机制与星形胶质细胞瘤有所不同。这些基因之间存在复杂的相互关系，有待进一步研究。
