Jin Lei, Wang Junxia, Zhu Guijin, Liu Qun
Reproductive Medicine Center, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei 430030, China.
Zhonghua Nan Ke Xue. 2004 Mar;10(3):227-8.
Intracytoplasmic sperm injection (ICSI) with sperm collected by percutaneous epididymal sperm aspiration (PESA) technique is nowadays the main treatment for obstructive azoospermia(OAS). To cryopreserve the epididymal sperm obstained by PESA can avoid repeat retrievals. We transferred the embryos fertilized by ICSI with frozen/thawed epididymal sperm and achieved a delivery, which shows that epididymal sperm obstained by PESA can be successfully cryopreserved.
经皮附睾精子抽吸术(PESA)采集精子后行卵胞浆内单精子注射(ICSI)是目前梗阻性无精子症(OAS)的主要治疗方法。冷冻保存经PESA获取的附睾精子可避免重复取精。我们移植了经冻融附睾精子ICSI受精的胚胎并获得分娩,这表明经PESA获取的附睾精子能够成功冷冻保存。