Traub R J, Monis P T, Robertson I, Irwin P, Mencke N, Thompson R C A
World Health Organization Collaborating Centre for the Molecular Epidemiology of Parasitic Infections, School of Veterinary and Biomedical Sciences, Murdoch University, South Street, Murdoch, Western Australia 6150.
Parasitology. 2004 Mar;128(Pt 3):253-62. doi: 10.1017/s0031182003004505.
Giardia duodenalis isolates recovered from humans and dogs living in the same locality in a remote tea-growing community of northeast India were characterized at 3 different loci; the SSU-rDNA, elongation factor 1-alpha (ef1-alpha) and triose phosphate isomerase (tpi) gene. Phylogenetic analysis of the SSU-rDNA and efl-alpha genes provided poor genetic resolution of the isolates within various assemblages, stressing the importance of using multiple loci when inferring genotypes to Giardia. Analysis of the tpi gene provided better genetic resolution and placed canine Giardia isolates within the genetic groupings of human isolates (Assemblages A and B). Further evidence for zoonotic transmission was supported by epidemiological data showing a highly significant association between the prevalence of Giardia in humans and presence of a Giardia-positive dog in the same household (odds ratio 3.01, 95% CI, 1.11, 8.39, P = 0.0000).
从印度东北部一个偏远茶叶种植社区同一地点生活的人类和犬类中分离出的十二指肠贾第虫，在3个不同基因座进行了特征分析；小亚基核糖体DNA（SSU-rDNA）、延伸因子1-α（ef1-α）和磷酸丙糖异构酶（tpi）基因。对SSU-rDNA和ef1-α基因的系统发育分析，在不同组合中对分离株的遗传分辨率较低，强调了在推断贾第虫基因型时使用多个基因座的重要性。tpi基因分析提供了更好的遗传分辨率，并将犬贾第虫分离株置于人类分离株的遗传分组（A组和B组）中。流行病学数据显示人类贾第虫患病率与同一家庭中存在贾第虫阳性犬之间存在高度显著关联（优势比3.01，95%可信区间，1.11，8.39，P = 0.0000），为人畜共患传播提供了进一步证据。
