Trombino Sonia, Serini Simona, Di Nicuolo Fiorella, Celleno Leonardo, Andò Sebastiano, Picci Nevio, Calviello Gabriella, Palozza Paola
Department of Pharmaceutical Sciences, University of Calabria, 87030 Arcavacata di Rende, Cosenza, Italy.
J Agric Food Chem. 2004 Apr 21;52(8):2411-20. doi: 10.1021/jf0303924.
Although an antioxidant mechanism has been involved in the beneficial effects of ferulic acid in human diseases, there are few reports on the antioxidant properties of this compound in isolated membranes and intact cells. Here, we evaluated the ability of ferulic acid in inhibiting lipid peroxidation in rat liver microsomal membranes and reactive oxygen species production in NIH-3T3 fibroblasts, induced by both tert-BOOH and AAPH. We also compared its antioxidant efficiency with that of other antioxidants, such as alpha-tocopherol, beta-carotene, and ascorbic acid, added alone or in combination. Ferulic acid acted as a potent antioxidant in our models, being more effective in protecting from tert-BOOH than from AAPH. Moreover, the compound was the most effective among the antioxidants tested. Synergistic interactions were observed when the compound was used in combination with the other antioxidants, suggesting that they can cooperate in preserving physiological integrity of cells exposed to free radicals.
尽管抗氧化机制与阿魏酸对人类疾病的有益作用有关，但关于该化合物在分离膜和完整细胞中的抗氧化特性的报道却很少。在此，我们评估了阿魏酸抑制叔丁基过氧化氢（tert-BOOH）和2,2'-偶氮二异丁基脒二盐酸盐（AAPH）诱导的大鼠肝微粒体膜脂质过氧化以及NIH-3T3成纤维细胞中活性氧生成的能力。我们还将其抗氧化效率与单独或联合添加的其他抗氧化剂（如α-生育酚、β-胡萝卜素和抗坏血酸）进行了比较。在我们的模型中，阿魏酸表现为一种有效的抗氧化剂，在保护细胞免受tert-BOOH损伤方面比AAPH更有效。此外，该化合物在所测试的抗氧化剂中最为有效。当该化合物与其他抗氧化剂联合使用时，观察到了协同相互作用，这表明它们可以协同保护暴露于自由基的细胞的生理完整性。
