Cortegano I, Civantos E, Aceituno E, del Moral A, López E, Lombardero M, del Pozo V, Lahoz C
Immunology Department, Fundación Jiménez Díaz, Madrid, Spain.
Allergy. 2004 May;59(5):485-90. doi: 10.1046/j.1398-9995.2003.00363.x.
This paper describes the cloning and expression of the Cupressus arizonica pollen protein Cup a 3. In addition, we present its modulation under polluted environmental conditions. Species of the Cupressaceae family are important because of their high sensitization prevalence.
Cup a 3 cloning is based on the sequence of the homologous protein Jun a 3. Cup a 3 was expressed with good yield in the methylotropic yeast Pichia pastoris.
Recombinant Cup a 3 (rCup a 3) contains 199 amino acids, 10 potential phosphorylation sites and no glycosylation sites. By immunoblot 63% of cypress allergic patients had specific immunoglobulin E antibodies against rCup a 3 (n = 104). This major allergen is homologous to members of the pathogenesis-related proteins (PR-5 group) and contributes to the overall allergenicity of C. arizonica pollen. Our results show that the increased expression of Cup a 3 is dependent on the pollution in the area where the pollen has been collected, being higher under polluted conditions.
Cup a 3 is a PR-5 protein derived from C. arizonica pollen. The expression of the protein under polluted conditions has a direct incidence on the pollen allergenicity, as has been demonstrated by skin tests and Radioallergosorbent test inhibition.
本文描述了亚利桑那柏花粉蛋白Cup a 3的克隆与表达。此外，我们还展示了其在污染环境条件下的调控情况。柏科植物因其高致敏率而具有重要意义。
Cup a 3的克隆基于同源蛋白Jun a 3的序列。Cup a 3在甲基营养型酵母毕赤酵母中以高产率表达。
重组Cup a 3（rCup a 3）包含199个氨基酸，10个潜在磷酸化位点且无糖基化位点。通过免疫印迹法，63%的柏树过敏患者对rCup a 3有特异性免疫球蛋白E抗体（n = 104）。这种主要过敏原与病程相关蛋白（PR - 5组）成员同源，并对亚利桑那柏花粉的整体致敏性有贡献。我们的结果表明，Cup a 3表达的增加取决于收集花粉地区的污染情况，在污染条件下表达更高。
Cup a 3是一种源自亚利桑那柏花粉的PR - 5蛋白。如皮肤试验和放射变应原吸附试验抑制所证明的，该蛋白在污染条件下的表达对花粉致敏性有直接影响。
