Liu Hongxia, Zhang Shusheng, Yu Ajuan, Qu Lingbo, Zhao Yufen, Huang Hongchun, Li Jichang
Chemistry Department, Key Laboratory of Chemical Biology and Organic Chemistry of Henan, Zhengzhou University, No. 75 Daxue Road, Zhengzhou 450052, PR China.
Bioorg Med Chem Lett. 2004 May 3;14(9):2339-44. doi: 10.1016/j.bmcl.2004.01.107.
The method for separation and determination of lactulose (L) and mannitol (M) in urine was developed by HPLC with a refractive index detector (RID). The linearity ranged from 5 to 1000 microg/mL for L and M, respectively. Recoveries ranged from 93.1% to 97.1%. The intra- and inter-day relative standard deviations of peak area were between 0.8-1.4% (n=3) and 1.4-3.6% (n=3). The limits of detection were obtained with 1.40 microg/mL for L and 1.65 microg/mL for M. The ratios of L/M in the urine samples for the spontaneous ascitic fluid infection (SAI), sterile ascitic fluid (SA) patients, and healthy volunteers (HV) were determined. The results showed well the correlations among the L/M ratio, intestinal permeability (IP) and the illness status of patients, and also indicated lactulose could improve the IP of SAI patients. The peaks of L and M in chromatograms were identified by electrospray ionization/mass spectrometry (ESI/MS), which ensured the accurate measurement of the ratio L/M. This method presented a rapid, accurate, and practical technique for determining IP in clinical practice and investigating the pathology of hepatocirrhosis.
采用高效液相色谱仪配示差折光检测器（RID）建立了尿液中乳果糖（L）和甘露醇（M）的分离测定方法。L和M的线性范围分别为5至1000μg/mL。回收率在93.1%至97.1%之间。峰面积的日内和日间相对标准偏差分别在0.8 - 1.4%（n = 3）和1.4 - 3.6%（n = 3）之间。L的检测限为1.40μg/mL，M的检测限为1.65μg/mL。测定了自发性腹水感染（SAI）患者、无菌性腹水（SA）患者和健康志愿者（HV）尿液样本中的L/M比值。结果很好地显示了L/M比值、肠道通透性（IP）与患者病情之间的相关性，还表明乳果糖可改善SAI患者的IP。通过电喷雾电离/质谱（ESI/MS）鉴定了色谱图中L和M的峰，从而确保了L/M比值的准确测定。该方法为临床实践中测定IP和研究肝硬化病理提供了一种快速、准确且实用的技术。
