微量人血清中赭曲霉毒素A的测定
Determination of Ochratoxin A in small volumes of human blood serum.
作者信息
Köller G, Rolle-Kampczyk U, Lehmann I, Popp P, Herbarth O
机构信息
Department of Human Exposure Research and Epidemiology, Centre for Environmental Research Leipzig-Halle, Permoserstr. 15, 04318 Leipzig, Germany.
出版信息
J Chromatogr B Analyt Technol Biomed Life Sci. 2004 May 25;804(2):313-7. doi: 10.1016/j.jchromb.2004.01.025.
A new simple and rapid method for analysing Ochratoxin A (OTA) in small volumes of human blood serum using capillary zone electrophoresis coupled to laser-induced fluorescence is described. The clean-up procedure solely consists of a double extraction step. To improve the reproducibility of migration times and quantification, two internal standards were used. The limit of detection was 0.55 ng/ml, with a linear range of 1-100 ng/ml of OTA in spiked human blood serum. The method is used to rapidly screen suspected patients.
描述了一种使用毛细管区带电泳结合激光诱导荧光分析少量人血清中赭曲霉毒素A（OTA）的新的简单快速方法。净化程序仅包括双重提取步骤。为提高迁移时间和定量的重现性，使用了两种内标。检测限为0.55 ng/ml，在加标人血清中OTA的线性范围为1-100 ng/ml。该方法用于快速筛查疑似患者。
Zotero 插件