Miño J, Moscatelli V, Hnatyszyn O, Gorzalczany S, Acevedo C, Ferraro G
Cátedra de Farmacología, Facultad de Farmacia y Bioquímica, Universidad de Buenos Aires, Junín 956 5do piso CP: 1113, Buenos Aires, Argentina.
Pharmacol Res. 2004 Jul;50(1):59-63. doi: 10.1016/j.phrs.2003.12.016.
The aqueous extract from aerial parts of Artemisia copa Phil. (Compositae), was evaluated for antinociceptive activity using writhing, formalin, and hot-plate tests in mice. A dose-related antinociceptive response was obtained in the writhing test at doses of 500 and 1000 mg/kg p.o. (percentage of inhibition 23.3 and 52.70, respectively). The extract also inhibited the second phase of formalin test (38.81%) and this effect was not antagonized by pretreatment with naloxone 5mg/kg i.p. Furthermore, no significant effect was obtained in the hot-plate test. Dichloromethane and ethanolic extracts, were analyzed for antiinflammatory activity with the carrageenan-induced paw edema in rats and the ear edema induced by 12-O-tetradecanoylphorbol-13 acetate (TPA) and arachidonic acid (AA) in mice. Both extracts showed antiinflammatory activity in the TPA (88 and 54%), and the ethanolic extract showed a 37% inhibition in AA test. No effects were seen at doses of 300 mg/kg p.o. and 100 mg/kg i.p. in the carrageenan test. The results obtained indicate that A. copa has analgesic and topical antiinflammatory activities that supports the folk medicinal use of the plant.
对毛莲蒿（菊科）地上部分的水提取物，在小鼠中通过扭体、福尔马林和热板试验评估其抗伤害感受活性。在扭体试验中，口服剂量为500和1000 mg/kg时获得了剂量相关的抗伤害感受反应（抑制百分比分别为23.3和52.70）。该提取物还抑制了福尔马林试验的第二阶段（38.81%），且腹腔注射5mg/kg纳洛酮预处理不能拮抗此作用。此外，在热板试验中未获得显著效果。用角叉菜胶诱导大鼠足爪水肿以及用12-O-十四烷酰佛波醇-13-乙酸酯（TPA）和花生四烯酸（AA）诱导小鼠耳水肿，分析二氯甲烷提取物和乙醇提取物的抗炎活性。两种提取物在TPA试验中均显示出抗炎活性（分别为88%和54%），乙醇提取物在AA试验中显示出37%的抑制率。在角叉菜胶试验中，口服剂量为300 mg/kg和腹腔注射剂量为100 mg/kg时未观察到效果。所得结果表明，毛莲蒿具有镇痛和局部抗炎活性，这支持了该植物的民间药用。
