Ben Miled Hanène, Saada Mariem, Jallali Ines, Ben Barka Zaineb, Tlili Mounira, Alimi Hichem, Sakly Mohsen, Ben Rhouma Khémais, Abderrabba Manef, Abdelmelek Hafedh, Tebourbi Olfa, Ksouri Riadh
Laboratoire des Plantes Aromatiques et Médicinales (LPAM), Centre de Biotechnologie, Technopôle de Borj Cédria (CBBC), BP 901, 2050, Hammam-Lif, Tunisie.
Laboratoire de Physiologie Intégrée, Faculté des Sciences de Bizerte, Université de Carthage, 7021, Jarzouna, Tunisie.
EXCLI J. 2017 Mar 31;16:439-447. doi: 10.17179/excli2016-735. eCollection 2017.
Rhus species are known in traditional medicine for their therapeutic virtue and their extracts showed numerous important properties including antimalarial, antimicrobial, antiviral, and hypoglycemic and anticonvulsant activities. Rhus tripartitum (Ucria) is a medicinal plant widely used in Tunisia folk medicine against chronic diarrhea and gastric ulcer. This study was designed to examine and antioxidant, anti-inflammatory and anticancer activities of four extracts of Rhus tripartitum root cortex with increasing solvent polarity (hexane, dichloromethane, methanol and water). HPLC was used to identify and quantify phenolic compounds in Rhus extract. Water extract showed the highest antioxidant activity using oxygen radical absorbance capacity (ORAC method) with 8.95 ± 0.47 µmol Trolox/mg and a cell based-assay with 0.28 ± 0.12 µmol Trolox/mg as compared to the other fractions. Moreover, methanol extract displayed the strongest anti-cancer activity against human lung carcinoma (A-549) and colon adenocarcinoma cell lines (DLD-1) with an IC value of 60.69 ± 2.58 and 39.83 ± 4.56 µg/ml (resazurin test) and 44.52 ± 5.96 and 55.65 ± 6.00 µg/ml (hoechst test), respectively. Besides, the highest anti-inflammatory activity, inhibiting nitric oxide (NO) release, was exhibited by dichloromethane extract with 31.5 % at 160 µg/ml in lipopolysaccharide (LPS)-stimulated RAW 264.7 macrophages. The HPLC analysis showed that catechol and kaempferol were the major phenolics. These data suggest the richness of all fractions of Ucria root on interesting bioactive molecules with different polarity and confirm the known traditional therapeutics virtues of this species for the treatment of dysentery, diarrhea and gastric ulcer.
盐肤木属植物在传统医学中因其治疗功效而闻名，其提取物显示出许多重要特性，包括抗疟疾、抗菌、抗病毒、降血糖和抗惊厥活性。三叶盐肤木是一种药用植物，在突尼斯民间医学中广泛用于治疗慢性腹泻和胃溃疡。本研究旨在检测三叶盐肤木根皮四种极性递增溶剂提取物（己烷、二氯甲烷、甲醇和水）的抗氧化、抗炎和抗癌活性。采用高效液相色谱法（HPLC）鉴定和定量盐肤木提取物中的酚类化合物。与其他组分相比，水提取物采用氧自由基吸收能力（ORAC法）显示出最高的抗氧化活性，为8.95±0.47 μmol Trolox/mg，基于细胞的检测为0.28±0.12 μmol Trolox/mg。此外，甲醇提取物对人肺癌（A-549）和结肠腺癌细胞系（DLD-1）显示出最强的抗癌活性，其半数抑制浓度（IC）值分别为60.69±2.58和39.83±4.56 μg/ml（刃天青试验）以及44.52±5.96和55.65±6.00 μg/ml（Hoechst试验）。此外, 二氯甲烷提取物在脂多糖（LPS）刺激的RAW 264.7巨噬细胞中表现出最高的抗炎活性，在160 μg/ml时抑制一氧化氮（NO）释放达31.5%。HPLC分析表明，儿茶酚和山奈酚是主要的酚类物质。这些数据表明三叶盐肤木根的所有组分富含具有不同极性的有趣生物活性分子，并证实了该物种在治疗痢疾、腹泻和胃溃疡方面已知的传统治疗功效。
