聚合酶会留下印记：分析大肠杆菌rpoB中的突变谱以评估聚合酶IV在自发突变中的作用。
Polymerases leave fingerprints: analysis of the mutational spectrum in Escherichia coli rpoB to assess the role of polymerase IV in spontaneous mutation.
作者信息
Wolff Erika, Kim Mandy, Hu Kaibin, Yang Hanjing, Miller Jeffrey H
机构信息
Department of Microbiology, Immunology, and Molecular Genetics, University of California-Los Angeles, 609 Charles E. Young #1602, Los Angeles, CA 90095, USA.
出版信息
J Bacteriol. 2004 May;186(9):2900-5. doi: 10.1128/JB.186.9.2900-2905.2004.
Abstract
We compared the distribution of mutations in rpoB that lead to rifampin resistance in strains with differing levels of polymerase IV (Pol IV), including strains with deletions of the Pol IV-encoding dinB gene, strains with a chromosomal copy of dinB, strains with the F'128 plasmid, and strains with plasmid amplification of either the dinB operon (dinB-yafNOP) or the dinB gene alone. This analysis identifies several hot spots specific to Pol IV which are virtually absent from the normal spontaneous spectrum, indicating that Pol IV does not contribute significantly to mutations occurring during exponential growth in liquid culture.
摘要
我们比较了不同聚合酶IV（Pol IV）水平的菌株中导致利福平耐药的rpoB基因突变分布情况，这些菌株包括缺失编码Pol IV的dinB基因的菌株、含有dinB染色体拷贝的菌株、含有F'128质粒的菌株，以及dinB操纵子（dinB - yafNOP）或单独dinB基因发生质粒扩增的菌株。该分析确定了几个特定于Pol IV的热点突变区域，这些区域在正常自发突变谱中几乎不存在，这表明Pol IV对液体培养指数生长期发生的突变贡献不大。
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本文引用的文献
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The dinB operon and spontaneous mutation in Escherichia coli.大肠杆菌中的dinB操纵子与自发突变
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