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采用藻类ATP生物测定微孔板技术对有色样品进行毒性测试。

Testing of coloured samples for toxicity by the algal-ATP bioassay microplate technique.

作者信息

Kwan K K

机构信息

Rivers Research Branch, National Water Research Institute, Canada Centre for Inland Waters, Burlington, Ontario, Canada L7R 4A6.

出版信息

Environ Pollut. 1989;60(1-2):47-53. doi: 10.1016/0269-7491(89)90219-4.

DOI:10.1016/0269-7491(89)90219-4
PMID:15092389
Abstract

Algal-ATP bioassay is a relatively simple, inexpensive, reliable and fast testing procedure for toxicity assessment. This algal growth inhibition bioassay technique using Selenastrum capricornutum was used to assess the toxicity levels in pulp and paper mill and thermomechanical mill effluent samples. Pulp and paper mill and thermomechanical mill effluent samples contain a wide range of colours due to certain physico-chemical properties of the test samples. The optical densities of the samples ranged from 0.27 to> 2.0 (a 600 nanometer). The colour variations in the test samples may interfere with ATP measurements. This paper presents data on the nature of interferences due to certain physico-chemical properties of the test sample and the usefulness of developed controls to overcome the problems for a more realistic toxicity assessment of coloured waste samples.

摘要

藻类ATP生物测定法是一种用于毒性评估的相对简单、廉价、可靠且快速的测试程序。这种使用羊角月牙藻的藻类生长抑制生物测定技术被用于评估造纸厂和热磨机废水样品中的毒性水平。由于测试样品的某些物理化学性质,造纸厂和热磨机废水样品含有多种颜色。样品的光密度范围为0.27至>2.0(600纳米)。测试样品中的颜色变化可能会干扰ATP测量。本文介绍了由于测试样品的某些物理化学性质导致的干扰性质的数据,以及所开发的对照对于克服问题以更实际地评估有色废物样品毒性的有用性。

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