Morcillo Y, Porte C
Environmental Chemistry Department, IIQAB-CSIC, Jordi Girona 18, 08034 Barcelona, Spain.
Environ Pollut. 2000 Jan;107(1):47-52. doi: 10.1016/s0269-7491(99)00133-5.
Clams--Ruditapes decussata--were transplanted to an organotin-polluted marina for a period up to 5 weeks. Tributyltin (TBT) was the major organotin compound detected in clams; TBT accumulation was extremely rapid, it reached a maximum tissue concentration of 290 ng/g wet weight 3 weeks after transplant, which represents a bioaccumulation factor of 12,000. Dibutyltin, monobutyltin and triphenyltin were also detected, although at a lesser extent (4-22 ng/g wet wt). Investigations on endogenous steroid content by radioimmunoassay revealed important changes in TBT-exposed clams. A 33% increase in testosterone titres was observed 5 weeks after transplant. Likewise, a time-dependent decrease in oestradiol levels was evidenced, viz. oestradiol titres decreased 2, 3.5 and 5-fold in clams sampled 1, 3 and 5 weeks after transplant, respectively. These observations together with previous data on TBT-exposure experiments suggest a potential masculinization of clam physiology as a consequence of TBT exposure.
将蛤仔（四角蛤蜊）移植到一个受有机锡污染的码头长达5周。三丁基锡（TBT）是在蛤仔中检测到的主要有机锡化合物；TBT积累极其迅速，移植3周后其组织浓度达到最大值290纳克/克湿重，这代表生物累积因子为12000。二丁基锡、一丁基锡和三苯基锡也被检测到，不过含量较少（4 - 22纳克/克湿重）。通过放射免疫分析法对内源类固醇含量进行的调查显示，暴露于TBT的蛤仔有显著变化。移植5周后观察到睾酮水平增加了33%。同样，雌二醇水平呈现出随时间下降的趋势，即在移植后1周、3周和5周采集的蛤仔中，雌二醇水平分别下降了2倍、3.5倍和5倍。这些观察结果以及之前关于TBT暴露实验的数据表明，由于TBT暴露，蛤仔生理机能可能出现雄性化。
