一种新型的具有嵌入金纳米团簇的介电膜的超高分辨率表面等离子体共振生物传感器。

A novel ultrahigh-resolution surface plasmon resonance biosensor with an Au nanocluster-embedded dielectric film.

作者信息

Hu W P, Chen S-J, Huang K-T, Hsu J H, Chen W Y, Chang G L, Lai K-A

机构信息

Institute of Biomedical Engineering, National Cheng Kung University, Tainan 701, Taiwan.

出版信息

Biosens Bioelectron. 2004 Jun 15;19(11):1465-71. doi: 10.1016/j.bios.2003.12.001.

DOI:10.1016/j.bios.2003.12.001

PMID:15093218

Abstract

The detection performance of conventional surface plasmon resonance (SPR) biosensors is limited to a 1 pg/mm(2) surface coverage of biomolecules, and consequently, such sensors struggle to detect the interaction of small molecules in low concentrations. The present study is attempted to propose the use of a novel SPR biosensor with Au nanoclusters embedded in a dielectric film to achieve a 10-fold improvement in the resolution performance. A co-sputtering method utilizing a multi-target sputtering system is used to fabricate the present dielectric films (SiO(2)) with embedded Au nanoclusters. It is shown that the sensitivity of the developed SPR biosensor can be improved by adjusting the size and volume fraction of the embedded Au nanoclusters in order to control the surface plasmon effect. The present gas detection and DNA hybridization experimental results confirm that the proposed Au nanocluster-enhanced SPR biosensor provides the potential to achieve an ultrahigh-resolution detection performance of approximately 0.1 pg/mm(2) surface coverage of biomolecules.

摘要

传统表面等离子体共振（SPR）生物传感器的检测性能局限于生物分子1 pg/mm²的表面覆盖量，因此，这类传感器难以检测低浓度小分子之间的相互作用。本研究试图提出使用一种新型的SPR生物传感器，该传感器在介电膜中嵌入金纳米团簇，以实现分辨率性能提高10倍。利用多靶溅射系统的共溅射方法来制备嵌入金纳米团簇的当前介电膜（SiO₂）。结果表明，通过调节嵌入金纳米团簇的尺寸和体积分数以控制表面等离子体效应，可以提高所开发的SPR生物传感器的灵敏度。当前的气体检测和DNA杂交实验结果证实，所提出的金纳米团簇增强型SPR生物传感器具有实现生物分子表面覆盖量约为0.1 pg/mm²的超高分辨率检测性能的潜力。