Esteban Maria Angeles, Rodríguez Alejandro, Ayala Alfonsa García, Meseguer José
Fish Innate Immune System Group, Department of Cell Biology, Faculty of Biology, University of Murcia, 30,100 Murcia, Spain.
Gen Comp Endocrinol. 2004 May 15;137(1):89-98. doi: 10.1016/j.ygcen.2004.02.006.
The effects of high doses of cortisol, biologically the most active corticosteroid in the circulating blood of teleost fish, on gilthead seabream (Sparus aurata L.) leucocytes were determined. Leucocytes isolated from the head-kidney (the principal haemopoietic organ of this fish species) were incubated with five different concentrations (ranging from 0 to 10(-1) mM) of cortisol for 30, 120, 240, 360, and 480 min and their effects on leucocyte viability and some of the main innate cellular immune responses were evaluated. The viability and the cytotoxic activity of leucocytes against tumor cells were not significantly affected by in vitro incubation with cortisol, at any of the assayed concentration or incubation times. With cortisol concentrations of 10(-9), 10(-7) or 10(-5) mM, the respiratory burst activity of head-kidney leucocytes were significantly depressed after 30 min of incubation. On the other hand, with cortisol concentrations of 10(-1) mM, the phagocytosis of yeast cells and the total peroxidase content of leucocytes were significantly depressed at incubation times longer than 240 min. To corroborate that the observed effects were due to the cortisol treatment, assays were developed using both cortisol and cycloheximide, which blocked the inhibitory effects of cortisol. The present results demonstrate that cortisol plays an important role in the down-regulation of phagocytic but not of cytotoxic activity in seabream leucocytes.
研究了高剂量皮质醇（硬骨鱼循环血液中生物学活性最高的皮质类固醇）对金头鲷（Sparus aurata L.）白细胞的影响。从头部肾脏（该鱼类的主要造血器官）分离的白细胞与五种不同浓度（范围从0至10^(-1) mM）的皮质醇孵育30、120、240、360和480分钟，并评估其对白细胞活力和一些主要先天性细胞免疫反应的影响。在任何测定浓度或孵育时间下，与皮质醇进行体外孵育对白细胞的活力和对肿瘤细胞的细胞毒性活性均无显著影响。在10^(-9)、10^(-7)或10^(-5) mM的皮质醇浓度下，孵育30分钟后，头部肾脏白细胞的呼吸爆发活性显著降低。另一方面，在10^(-1) mM的皮质醇浓度下，孵育时间超过240分钟时，酵母细胞的吞噬作用和白细胞的总过氧化物酶含量显著降低。为了证实观察到的效应是由于皮质醇处理所致，使用皮质醇和环己酰亚胺进行了试验，环己酰亚胺可阻断皮质醇的抑制作用。目前的结果表明，皮质醇在下调金头鲷白细胞的吞噬活性而非细胞毒性活性方面发挥重要作用。
