Salinas Irene, Rodríguez Alejandro, Esteban M Angeles, Meseguer José
Department of Cell Biology, Faculty of Biology, University of Murcia, 30100 Murcia, Spain.
Dev Comp Immunol. 2006;30(3):325-34. doi: 10.1016/j.dci.2005.05.001. Epub 2005 May 31.
The possible immuneregulatory effects of two purine nucleotides, adenosine and cyclohexyladenosine, on gilthead seabream (Sparus aurata L.) leucocytes were investigated. Leucocytes isolated from the head-kidney were incubated with five different concentrations (ranging from 0 to 100 microM) of adenosine or cyclohexyladenosine for 30, 180 and 360 min and the effect on leucocyte viability and some of the main innate cellular immune responses was evaluated. Adenosine did not significantly affect the viability or the innate immune parameters of seabream leucocytes at any of the assayed concentrations or incubation times. High concentrations of cyclohexyladenosine, on the other hand, caused a significant drop in the respiratory burst activity of head-kidney leucocytes at all the times assayed. In addition, phagocytosis of yeast cells was significantly rapidly depressed (30 min) after incubation with 100 microM of this analogue. The present results demonstrate that cyclohexyladenosine at high concentrations is capable of down-regulating certain innate immune responses in seabream leucocytes, suggesting that telesot fish immune cells, like their mammal counterparts, possess receptors for purine nucleotides.
研究了两种嘌呤核苷酸，即腺苷和环己基腺苷，对金头鲷（Sparus aurata L.）白细胞可能的免疫调节作用。将从头肾分离的白细胞与五种不同浓度（0至100 microM）的腺苷或环己基腺苷孵育30、180和360分钟，并评估其对白细胞活力和一些主要先天性细胞免疫反应的影响。在任何测定浓度或孵育时间下，腺苷均未显著影响金头鲷白细胞的活力或先天性免疫参数。另一方面，在所有测定时间，高浓度的环己基腺苷均导致头肾白细胞的呼吸爆发活性显著下降。此外，与100 microM的这种类似物孵育后，酵母细胞的吞噬作用在30分钟时显著迅速降低。目前的结果表明，高浓度的环己基腺苷能够下调金头鲷白细胞中的某些先天性免疫反应，这表明硬骨鱼免疫细胞与哺乳动物免疫细胞一样，具有嘌呤核苷酸受体。
