Apaydin R, Gürbüz Y, Bayramgürler D, Müezzinoglu B, Bilen N
Department of Dermatology, Medical Faculty, Kocaeli University, 41 900 Kocaeli, Turkey.
J Eur Acad Dermatol Venereol. 2004 May;18(3):305-9. doi: 10.1111/j.1468-3083.2004.00905.x.
To understand the role of epidermal cells in the pathogenesis of lichen amyloidosus (LA) and macular amyloidosis (MA).
We carried out immunohistochemical investigations on cytokeratins (CKs) in amyloid deposits in formalin-fixed and paraffin-embedded tissue specimens from eight persons with LA and 12 with MA. The primary antibodies of CK1-8 (AE3), CK10 (DEK-10), CK14 (LL002), CK17 (E3), CK18 (DC10), CK19 (KS19.1), CK5/6/18 (LP34) and CK8/18 (5D3) were used in the study.
In amyloid deposits, immunoreactivity with only two monoclonal antibodies (CK1-8 and CK5/6/18) was observed in 14 cases (eight LA and six MA), confirming the hypothesis that epidermal cells participate in amyloid formation of LA and MA.
All of the CKs detected in amyloid deposits were basic type (type II). It seems plausible either that acidic CKs might be degraded faster than basic types in amyloidogenesis or that paraffin-embedded tissue specimens are less sensitive than frozen tissue sections. The results of our study suggest that when paraffin-embedded specimens are investigated by immunohistochemical methods, CK5 antibody is useful in the diagnosis of LA and MA.
了解表皮细胞在苔藓样淀粉样变（LA）和斑状淀粉样变（MA）发病机制中的作用。
我们对8例LA患者和12例MA患者经福尔马林固定、石蜡包埋的组织标本中的淀粉样沉积物进行细胞角蛋白（CKs）免疫组化研究。研究中使用了CK1 - 8（AE3）、CK10（DEK - 10）、CK14（LL002）、CK17（E3）、CK18（DC10）、CK19（KS19.1）、CK5/6/18（LP34）和CK8/18（5D3）的一抗。
在淀粉样沉积物中，14例（8例LA和6例MA）仅观察到与两种单克隆抗体（CK1 - 8和CK5/6/18）的免疫反应性，证实了表皮细胞参与LA和MA淀粉样蛋白形成的假说。
在淀粉样沉积物中检测到的所有CKs均为碱性类型（II型）。在淀粉样蛋白生成过程中，酸性CKs可能比碱性类型降解得更快，或者石蜡包埋的组织标本比冰冻组织切片敏感性更低，这两种情况似乎都有道理。我们的研究结果表明，当通过免疫组化方法研究石蜡包埋标本时，CK5抗体对LA和MA的诊断有用。
