Huilgol S C, Ramnarain N, Carrington P, Leigh I M, Black M M
St John's Institute of Dermatology, St Thomas' Hospital, London, United Kingdom.
Australas J Dermatol. 1998 May;39(2):81-5. doi: 10.1111/j.1440-0960.1998.tb01253.x.
The expression of keratins was investigated immunohistochemically on formalin-fixed and snap-frozen primary cutaneous amyloidosis tissue with a panel of monospecific and polyspecific antikeratin antibodies, with recognized keratins K1, K5, K6, K7, K8, K10, K14, K16, K17, K18 and K19. Amyloid deposits in frozen sections of seven cases of macular amyloidosis and lichen amyloidosus always reacted with antibodies LP34 (labelling K5, K6 and K18), MNF 116 (labelling K5, K6, K8, K10, K17 and K18), and RCK 102 (labelling K5 and K8); frozen sections in one case each of the seven cases also reacted with antibodies LL001 (labelling K14), LP1K (labelling K7 and K17), and LP2K (labelling K19). In formalin-fixed sections of 13 cases of macular amyloidosis and lichen amyloidosus, amyloid deposits were labelled with LP34 in three sections, MNF 116 in four sections, LL020 (labelling keratins K5 and K6) in one section, and LP2K in two sections. In nodular primary cutaneous amyloidosis, amyloid deposits were not labelled with any antikeratin antibodies. These data confirm that amyloid in macular amyloidosis and lichen amyloidosus contains keratin epitopes, and suggests derivation of the fibrillar component from keratin intermediate filaments. Several different keratins appear to undergo conversion to amyloid. LP34, MNF 116 and RCK 102 antibodies, which have in common the labelling of keratin K5, may be useful in the diagnosis of macular and papular amyloidosis with frozen tissue sections.
使用一组单特异性和多特异性抗角蛋白抗体，对福尔马林固定和速冻的原发性皮肤淀粉样变组织进行免疫组织化学研究，这些抗体可识别角蛋白K1、K5、K6、K7、K8、K10、K14、K16、K17、K18和K19。7例斑状淀粉样变和苔藓样淀粉样变冰冻切片中的淀粉样沉积物始终与LP34抗体（标记K5、K6和K18）、MNF 116抗体（标记K5、K6、K8、K10、K17和K18）以及RCK 102抗体（标记K5和K8）发生反应；7例中的各1例冰冻切片还与LL001抗体（标记K14）、LP1K抗体（标记K7和K17）以及LP2K抗体（标记K19）发生反应。在13例斑状淀粉样变和苔藓样淀粉样变的福尔马林固定切片中，3例切片中的淀粉样沉积物被LP34标记，4例被MNF 116标记，1例被LL020抗体（标记角蛋白K5和K6）标记，2例被LP2K标记。在结节性原发性皮肤淀粉样变中，淀粉样沉积物未被任何抗角蛋白抗体标记。这些数据证实，斑状淀粉样变和苔藓样淀粉样变中的淀粉样物质含有角蛋白表位，并提示纤维成分源自角蛋白中间丝。几种不同的角蛋白似乎会转化为淀粉样物质。共同标记角蛋白K5的LP34、MNF 116和RCK 102抗体，可能有助于利用冰冻组织切片诊断斑状和丘疹性淀粉样变。
