严重急性呼吸综合征（SARS）相关冠状病毒感染过程中的抗体反应和病毒血症。
Antibody response and viraemia during the course of severe acute respiratory syndrome (SARS)-associated coronavirus infection.
作者信息
Chen Weijun, Xu Zuyuan, Mu Jingsong, Yang Ling, Gan Haixue, Mu Feng, Fan Baoxing, He Bo, Huang Shengyong, You Bo, Yang Yongkui, Tang Xiangjun, Qiu Ling, Qiu Yan, Wen Jie, Fang Jianqiu, Wang Jian
机构信息
Beijing Genomics Institute, Chinese Academy of Sciences, Beijing 101300, China 2Beijing BGI-GBI Biotech Co. Ltd, Beijing 101300, China 3Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China 4Beijing 302 Hospital, Beijing 100039, China 5Beijing 301 Hospital, Beijing 100853, China 6Beijing Union Hospital, Beijing 100730, China 7Beijing Red Cross Blood Center, Beijing 100088, China.
出版信息
J Med Microbiol. 2004 May;53(Pt 5):435-438. doi: 10.1099/jmm.0.45561-0.
To understand the time-course of viraemia and antibody responses to severe acute respiratory syndrome-associated coronavirus (SARS-CoV), RT-PCR and ELISA were used to assay 376 blood samples from 135 SARS patients at various stages of the illness, including samples from patients who were in their early convalescent phase. The results showed that IgM antibodies decreased and became undetectable 11 weeks into the recovery phase. IgG antibodies, however, remained detectable for a period beyond 11 weeks and were found in 100 % of patients in the early convalescent phase. SARS-CoV viraemia mainly appeared 1 week after the onset of illness and then decreased over a period of 1 month, becoming undetectable in the blood samples of the convalescent patients. At the peak of viraemia, viral RNA was detectable in 75 % of blood samples from patients who were clinically diagnosed with SARS 1 or 2 weeks before the test.
为了解严重急性呼吸综合征相关冠状病毒（SARS-CoV）病毒血症和抗体反应的时间进程，采用逆转录聚合酶链反应（RT-PCR）和酶联免疫吸附测定（ELISA）对135例处于疾病不同阶段的SARS患者的376份血样进行检测，包括处于恢复期早期患者的样本。结果显示，IgM抗体在恢复期第11周时下降并变得无法检测到。然而，IgG抗体在11周后仍可检测到，且在恢复期早期100%的患者中均可发现。SARS-CoV病毒血症主要在发病1周后出现，然后在1个月内下降，在恢复期患者的血样中变得无法检测到。在病毒血症高峰期，在检测前1或2周临床诊断为SARS的患者75%的血样中可检测到病毒RNA。
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