Gurfinkel Michael, Ke Shi, Wen Xiaoxia, Li Chun, Sevick-Muraca Eva M
Department of Chemical Engineering, Texas A&M University, College Station, TX 77843-3122, USA.
Dis Markers. 2003;19(2-3):107-21. doi: 10.1155/2004/474818.
The advent of recent advances in near-infrared laser diodes and fast electro-optic detection has spawned a new research field of diagnostic spectroscopy and imaging based on targeting and reporting exogenous fluorescent agents. This review seeks to concisely address the physics, instrumentation, advancements in tomography, and near-infrared fluorescent contrast agent development that promises selective and specific molecular targeting of diseased tissues. As an example of one area of the field, recent work focusing on pharmacokinetic analysis of fluorophores targeting the epidermal growth factor receptor (EGFR) is presented in a human breast cancer xenograft mouse model to demonstrate specificity of molecularly targeted contrast agents. Finally, a critical evaluation of the limitations and the opportunities for future translation of fluorescence-enhanced optical imaging of deep tissues is presented.
近年来近红外激光二极管和快速电光检测技术的进步催生了一个基于靶向和报告外源性荧光剂的诊断光谱学与成像新研究领域。本综述旨在简要阐述物理学、仪器设备、断层扫描技术进展以及近红外荧光造影剂的开发情况，这些有望实现对病变组织的选择性和特异性分子靶向。作为该领域一个方面的示例，在人乳腺癌异种移植小鼠模型中展示了近期针对靶向表皮生长因子受体（EGFR）的荧光团进行药代动力学分析的工作，以证明分子靶向造影剂的特异性。最后，对深部组织荧光增强光学成像的局限性以及未来转化应用的机会进行了批判性评估。
