Hahnel D, Beyer K, Engelmann B
Physiologisches Institut der Universität München, Germany.
Free Radic Biol Med. 1999 Nov;27(9-10):1087-94. doi: 10.1016/s0891-5849(99)00142-2.
The recently discovered peroxyl radical scavenging properties of plasmalogen phospholipids led us to evaluate their potential interactions with alpha-tocopherol. The oxidative decay of plasmalogen phospholipids and of polyunsaturated fatty acids as induced by peroxyl radicals (generated from 2,2'-azobis-2-amidinopropane hydrochloride; AAPH) was studied in micelles using 1H-NMR and chemical analyses. In comparison with alpha-tocopherol, a 20- to 25-fold higher concentration of plasmalogen phospholipids was needed to induce a similar inhibition of peroxyl radical-mediated oxidation of polyunsaturated fatty acids. Plasmalogen phospholipids and alpha-tocopherol protected each other from oxidative degradation. In low-density lipoproteins (LDL) and micelles supplemented with plasmalogen phospholipids plus alpha-tocopherol, the peroxyl radical-promoted oxidation was additively diminished. The differences in the capacities to inhibit oxidation processes induced by peroxyl radicals between the plasmalogen phospholipids and alpha-tocopherol were less pronounced in the LDL particles than in the micelles. In conclusion, plasmalogen phospholipids and alpha-tocopherol apparently compete for the interaction with the peroxyl radicals. Oxidation processes induced by peroxyl radicals are inhibited in an additive manner in the presence of the two radical scavengers. The contribution of the plasmalogen phospholipids to the protection against peroxyl radical promoted oxidation in vivo is expected to be at least as important as that of alpha-tocopherol.
最近发现的缩醛磷脂的过氧自由基清除特性促使我们评估它们与α-生育酚的潜在相互作用。使用1H-NMR和化学分析方法，研究了由2,2'-偶氮二（2-脒基丙烷）盐酸盐（AAPH）产生的过氧自由基诱导的缩醛磷脂和多不饱和脂肪酸在胶束中的氧化衰变。与α-生育酚相比，需要高20至25倍浓度的缩醛磷脂才能对过氧自由基介导的多不饱和脂肪酸氧化产生类似的抑制作用。缩醛磷脂和α-生育酚相互保护免受氧化降解。在补充了缩醛磷脂加α-生育酚的低密度脂蛋白（LDL）和胶束中，过氧自由基促进的氧化作用呈加和性减弱。缩醛磷脂和α-生育酚在抑制过氧自由基诱导的氧化过程的能力上的差异在LDL颗粒中不如在胶束中明显。总之，缩醛磷脂和α-生育酚显然竞争与过氧自由基的相互作用。在两种自由基清除剂存在下，过氧自由基诱导的氧化过程以加和方式受到抑制。预计缩醛磷脂在体内对过氧自由基促进的氧化的保护作用至少与α-生育酚一样重要。
