Petty Jeffrey T, Zheng Jie, Hud Nicholas V, Dickson Robert M
School of Chemistry and Biochemistry, Georgia Institute of Technology, Atlanta, Georgia 30332-0400 USA.
J Am Chem Soc. 2004 Apr 28;126(16):5207-12. doi: 10.1021/ja031931o.
The high affinity of Ag+ for DNA bases has enabled creation of short oligonucleotide-encapsulated Ag nanoclusters without formation of large nanoparticles. Time-dependent formation of cluster sizes ranging from Ag1 to Ag4/oligonucleotide were observed with strong, characteristic electronic transitions between 400 and 600 nm. The slow nanocluster formation kinetics enables observation of specific aqueous nanocluster absorptions that evolve over a period of 12 h. Induced circular dichroism bands confirm that the nanoclusters are associated with the chiral ss-DNA template. Fluorescence, absorption, mass, and NMR spectra all indicate that multiple species are present, but that their creation is both nucleotide- and time-dependent.
银离子对DNA碱基的高亲和力使得能够创建短寡核苷酸包裹的银纳米团簇，而不会形成大的纳米颗粒。观察到从Ag1到Ag4/寡核苷酸的团簇尺寸随时间的形成过程，在400至600纳米之间有强烈的特征性电子跃迁。缓慢的纳米团簇形成动力学使得能够观察到特定的水性纳米团簇吸收，这种吸收在12小时内逐渐演变。诱导圆二色性带证实纳米团簇与手性单链DNA模板相关。荧光、吸收、质谱和核磁共振光谱均表明存在多种物种，但其形成既依赖于核苷酸，也依赖于时间。
