Rettigner Chantal, Leclipteux Thierry, De Meerschman François, Focant Charles, Losson Bertrand
Laboratory of Parasitology and Pathology of Parasitic Diseases, Department of Infectious and Parasitic Diseases, Faculty of Veterinary Medicine, University of Liege, Boulevard de Colonster, 20, 4000 Liege, Belgium.
Vet Res. 2004 Mar-Apr;35(2):225-32. doi: 10.1051/vetres:2004005.
The present work compared inbred (CBA/Ca) and outbred (Swiss white) strains of mice for their capacity to cope with a Neospora caninum infection and to consistently produce tissue cysts. In each experiment Swiss white and CBA/Ca mice were given three different doses of NC-1 tachyzoites. Lymphoproliferative and humoral responses as well as cytokine production were evaluated eight weeks after infection (PI) whereas tissue cyst production and histopathology were assessed 4, 6 and 10 weeks PI in immunosuppressed mice. Tissue cysts were observed 10 weeks after infection only in CBA/Ca mice receiving the two highest inoculum doses. Furthermore this strain showed the highest specific lymphoproliferative response. A mixed cytokine response with elevated IFN-gamma and fairly low IL-4 and IL-10 secretion was recorded. In both strains, no lesions were observed in the tissues of infected mice. This study indicates that CBA/Ca female mice infected with 5 x 10(6) NC-1 tachyzoites represent a useful model for the study of specific maternal immune responses in pregnant animals.
本研究比较了近交系（CBA/Ca）和远交系（瑞士白化）小鼠应对犬新孢子虫感染并持续产生组织包囊的能力。在每个实验中，给瑞士白化小鼠和CBA/Ca小鼠接种三种不同剂量的NC-1速殖子。在感染后8周（PI）评估淋巴细胞增殖和体液反应以及细胞因子产生情况，而在免疫抑制小鼠中于感染后4、6和10周评估组织包囊产生和组织病理学情况。仅在接受两种最高接种剂量的CBA/Ca小鼠中，感染10周后观察到组织包囊。此外，该品系表现出最高的特异性淋巴细胞增殖反应。记录到混合细胞因子反应，其中干扰素-γ升高，白细胞介素-4和白细胞介素-10分泌相当低。在两个品系中，感染小鼠的组织均未观察到病变。本研究表明，感染5×10⁶个NC-1速殖子的CBA/Ca雌性小鼠是研究怀孕动物特异性母体免疫反应的有用模型。
