Ushio-Watanabe Nanako, Fujihara Rio, Watanabe Kenichi, Yamada Manabu, Kobayashi Yoshiyasu, Nishikawa Yoshifumi
National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Hokkaido 080-8555, Japan.
Kochi Seibu Livestock Hygiene Service Center, Kochi 786-0043, Japan.
Microorganisms. 2025 Aug 22;13(9):1970. doi: 10.3390/microorganisms13091970.
, as well as , secrete proteins that facilitate the invasion of host cells and the regulation of host immune response and metabolism. However, the localization of the secretory proteins in infected animal brains has not been studied in detail. Here, we investigate the brain and intracellular distribution of the secretory proteins in experimentally infected mice and naturally infected calves through histopathology and immunohistochemistry (IHC) to detect surface antigen 1 (NcSAG1), cyclophilin (NcCYP), profilin (NcPF), dense granule protein 6 (NcGRA6), and NcGRA7. These methods revealed that numerous tachyzoites positive for NcSAG1, NcCYP, NcPF, NcGRA6, and NcGRA7 were localized in and around the animals' necrotic lesions, and NcGRA7 was diffusely observed in the necrotic lesions of the infected mice. Moreover, IHC revealed that NcGRA6 and NcGRA7 were distributed in the cytoplasm of infected neurons around the parasites in the infected mice and calves. This suggests that NcGRA6 and NcGRA7 might be directly related to the alteration of neuronal metabolism and activity, and that NcGRA7 might be related to the formation of necrotic lesions.
以及 ，分泌促进宿主细胞侵袭、宿主免疫反应调节和新陈代谢的蛋白质。然而，分泌蛋白在受感染动物大脑中的定位尚未得到详细研究。在这里，我们通过组织病理学和免疫组织化学（IHC）研究实验感染小鼠和自然感染小牛中分泌蛋白的脑内和细胞内分布，以检测表面抗原1（NcSAG1）、亲环蛋白（NcCYP）、肌动蛋白结合蛋白（NcPF）、致密颗粒蛋白6（NcGRA6）和NcGRA7。这些方法显示，大量NcSAG1、NcCYP、NcPF、NcGRA6和NcGRA7呈阳性的速殖子位于动物坏死病变内及其周围，并且在感染小鼠的坏死病变中广泛观察到NcGRA7。此外，免疫组织化学显示，NcGRA6和NcGRA7分布在感染小鼠和小牛体内寄生虫周围受感染神经元的细胞质中。这表明NcGRA6和NcGRA7可能与神经元代谢和活性的改变直接相关，并且NcGRA7可能与坏死病变的形成有关。
