成纤维细胞形态对胶体光刻法制备的纳米柱的响应变化。

Changes in fibroblast morphology in response to nano-columns produced by colloidal lithography.

作者信息

Dalby Matthew John, Riehle Mathis O, Sutherland Duncan S, Agheli Hossein, Curtis Adam S G

机构信息

Centre for Cell Engineering Institute of Biomedical and Life Sciences, University of Glasgow, Joseph Black Building, Glasgow G12 8QQ, UK.

出版信息

Biomaterials. 2004 Oct;25(23):5415-22. doi: 10.1016/j.biomaterials.2003.12.049.

DOI:10.1016/j.biomaterials.2003.12.049

PMID:15130726

Abstract

In designing new biomaterials, specific chemical and topographical cues will be important in guiding cell response. Filopodia are actin-driven structures produced by cells and speculated to be involved in cell sensing of the three-dimensional environment. This report quantifies filopodia response to cylindrical nano-columns (100 nm diameter, 160 nm high) produced by colloidal lithography. Also observed were actin cytoskeleton morphology by fluorescence microscopy and filopodia morphology by electron microscopy (scanning and transmission). The results showed that the fibroblasts used produced more filopodia per microm of cell perimeter and that filopodia could often be seen to interact with the cells' nano-environment. By understanding as to which features evoke spatial reactions in cells, it may be possible to design better biomaterials.

摘要

在设计新型生物材料时，特定的化学和拓扑线索对于引导细胞反应至关重要。丝状伪足是由细胞产生的肌动蛋白驱动结构，据推测参与细胞对三维环境的感知。本报告量化了丝状伪足对通过胶体光刻法制备的圆柱形纳米柱（直径100纳米，高160纳米）的反应。还通过荧光显微镜观察了肌动蛋白细胞骨架形态，并通过电子显微镜（扫描和透射）观察了丝状伪足形态。结果表明，所用的成纤维细胞每微米细胞周长产生更多的丝状伪足，并且经常可以看到丝状伪足与细胞的纳米环境相互作用。通过了解哪些特征会引起细胞中的空间反应，有可能设计出更好的生物材料。