Kudo Takahiko, Nurgalieva Zhannat Z, Conner Margaret E, Crawford Sue, Odenbreit Stefan, Haas Rainer, Graham David Y, Yamaoka Yoshio
Department of Medicine, Baylor College of Medicine, Houston, Texas, USA.
J Clin Microbiol. 2004 May;42(5):2279-81. doi: 10.1128/JCM.42.5.2279-2281.2004.
Polyclonal antisera to either a synthetic OipA peptide or a recombinant OipA protein detected OipA expression in Helicobacter pylori and correlated with functional oipA status determined by PCR sequence (sensitivity and specificity of >94%). Immunoblotting is a simple and accurate method for detecting expression of the important virulence factor OipA.
针对合成的OipA肽或重组OipA蛋白的多克隆抗血清可检测幽门螺杆菌中的OipA表达，且与通过PCR序列确定的功能性oipA状态相关（敏感性和特异性>94%）。免疫印迹是检测重要毒力因子OipA表达的一种简单而准确的方法。
Zotero 插件